Extracellular nucleic acids

  title={Extracellular nucleic acids},
  author={Valentin V. Vlassov and P. P. Laktionov and Elena Yu. Rykova},
Extracellular nucleic acids are found in different biological fluids in the organism and in the environment: DNA is a ubiquitous component of the organic matter pool in the soil and in all marine and freshwater habitats. Data from recent studies strongly suggest that extracellular DNA and RNA play important biological roles in microbial communities and in higher organisms. DNA is an important component of bacterial biofilms and is involved in horizontal gene transfer. In recent years, the… 

Extracellular Nucleic Acids of the Marine Phototrophic Bacterium Rhodovulum sulfidophilum and Related Bacteria: Physiology and Biotechnology

This chapter describes highly efficient extracellular nucleic acid production of Rhodovulum sp.

Cell-free and cell-bound circulating nucleic acid complexes: mechanisms of generation, concentration and content

Recent studies provide evidence of the circulating nucleome organization complexity indicating that discovery of extracellular DNA generation and circulation patterns in healthy condition and cancer is essential to enable the development of proper approaches for the selection of valid diagnostic markers.

The Biology of Circulating Nucleic Acids in Plasma and Serum (CNAPS)

The relationship of the DNA from cancer patients is considered with respect to the formation of metastases and the amounts of DNA/RNA present together with the variables affecting these amounts are discussed.

Characterization of extracellular DNA production and flocculation of the marine photosynthetic bacterium Rhodovulum sulfidophilum

It seems likely that the extracellular soluble DNA observed in the rich medium may be incorporated into the large floc and play a role in floc maintenance in poor medium, after addition of an inhibitor of quorum sensing, α-cyclodextrin, inhibited huge floc Maintenance in the nutritionally poor medium.

Characterization of cellular and extracellular DNA in saliva.

The Biology of CNAPS

The relationship of the DNA from cancer patients is considered with respect to the formation of metastases and the amounts of DNA/RNA present together with the variables affecting these amounts are discussed.

Circulating Nucleic Acids in Health and Disease

The usefulness of serological DNA and RNA markers in diagnosis and prognosis of cancer and other pathological states, including trauma, stroke, myocardial infarction, hematological disorders, complications in organ transplantation, and diabetes are discussed.

Biofilms can be dispersed by focusing the immune system on a common family of bacterial nucleoid-associated proteins

It is shown that the DNABII family of proteins was also critical for the integrity of the EPS matrix of biofilms that contain eDNA, and this methodology to debulk the biofilm of EPS was shown to work synergistically with otherwise ineffective traditional anti-microbial approaches in vitro.

Pseudomonas aeruginosa produces an extracellular deoxyribonuclease that is required for utilization of DNA as a nutrient source.

It is shown that P. aeruginosa is capable of using DNA as a nutrient source under phosphate-limiting conditions, or when DNA is supplied as a source of phosphate, expression of PA3909 is induced.

Extracellular DNA: A Nutritional Trigger of Mycoplasma bovis Cytotoxicity

Extracellular DNA was identified as a limiting nutrient for mycoplasma proliferation under cell culture conditions and the growth-promoting effect induced by supplementation with eDNA was associated with important cytotoxicity for actively dividing host cells, but not confluent monolayers.

Circulating nucleic acids in higher organisms.

Simultaneous Recovery of Extracellular and Intracellular DNA Suitable for Molecular Studies from Marine Sediments

This study developed an efficient procedure to recover simultaneously DNA associated with microbial cells and extracellular DNA from the same sediment sample, and obtained evidence that extrace cellular DNA recovered from different sediments did not contain amplifiable 16S rRNA genes.

Extracellular Nucleic Acids in Cultures of Long‐Term Cultivated Eukaryotic Cells

Investigation of the kinetics of nucleic acid release by HeLa and A431 cells shows characteristic patterns of cell‐associated and free RNA and DNA molecules, and electrophoretic analysis reveals characteristic patterns.

Extracellular DNA and RNA produced by a marine photosynthetic bacterium Rhodovulum sulfidophilum.

It is revealed that the extracellular nucleic acids of Rhodovulum sulfidophilum are a mixture of double-Stranded DNAs and single-stranded RNAs, and at least a part of these DNAs is probably an amplified portion of genomic DNA.

Proteins involved in binding and cellular uptake of nucleic acids

The methods for revealing and dentification of proteins, possible mechanisms of protein-mediated transport of nucleic acids, and cellular functions of these proteins are described.

Extracellular DNA in Single- and Multiple-Species Unsaturated Biofilms

It is concluded that extracellular DNA production in unsaturated biofilms is species dependent and that the phylogenetic information contained in this DNA pool is quantifiable and distinct from either total or cellular DNA.

Circulating DNA in higher organisms cancer detection brings back to life an ignored phenomenon.

In this work, nude mice injected with DNA excreted by antigen stimulated human T lymphocytes produced specific antibodies expressing human characteristics, and the fact that living cells spontaneously release DNA within a homeostatic mechanism is outlined.

Cellular Uptake of Deoxyribonucleic Acid by Human Tissue Culture Cells

M Mammalian tissue culture in conjunction with the use of labelled homologous deoxyribonucleic acid offers a system in which the possibilities of extracellular transfer of de oxygenribon nucleic acid in higher forms can be investigated.

Extracellular DNA Plays a Key Role in Deep-Sea Ecosystem Functioning

It is demonstrated that extracellular DNA accounts for about one fifth of the total organic phosphorus regeneration and provides almost half of the prokaryotic demand for organic phosphorus in deep-sea ecosystem functioning on a global scale.