Extracellular monoenzyme deglycosylation system of 7-O-linked flavonoid β-rutinosides and its disaccharide transglycosylation activity from Stilbella fimetaria

@article{Mazzaferro2010ExtracellularMD,
  title={Extracellular monoenzyme deglycosylation system of 7-O-linked flavonoid $\beta$-rutinosides and its disaccharide transglycosylation activity from Stilbella fimetaria},
  author={Laura S Mazzaferro and Lucrecia Pi{\~n}uel and Marisol Minig and Javier D. Breccia},
  journal={Archives of Microbiology},
  year={2010},
  volume={192},
  pages={383-393}
}
We screened for microorganisms able to use flavonoids as a carbon source; and one isolate, nominated Stilbella fimetaria SES201, was found to possess a disaccharide-specific hydrolase. It was a cell-bound ectoenzyme that was released to the medium during conidiogenesis. The enzyme was shown to cleave the flavonoid hesperidin (hesperetin 7-O-α-rhamnopyranosyl-β-glucopyranoside) into rutinose (α-rhamnopyranosyl-β-glucopyranose) and hesperetin. Since only intracellular traces of monoglycosidase… 
A novel Aspergillus oryzae diglycosidase that hydrolyzes 6-O-α-L-rhamnosyl-β-D-glucoside from flavonoids
Abstractα-L-Rhamnosyl-β-D-glucosidase (rutinosidase) hydrolyzes the glycosidic linkage between the disaccharide 6-O-α-L-rhamnosyl-β-D-glucoside (rutinose) and the aglycone. We identified a
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TLDR
Findings demonstrate that organisms other than plants and filamentous fungi can contribute to an expansion of the diglycosidase toolbox.
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TLDR
The utility of the recombinant rutinosidase was demonstrated by its use for the synthesis of a broad spectrum of rutinoides of primary, secondary, acyclic and phenolic alcohols as well as for the preparation of free rutinose.
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TLDR
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TLDR
The aroma precursors were significantly hydrolyzed, which indicated the potential use of the enzyme for biotechnological applications, for example, in aroma modulation of fermented foods.
Hydrolysis of flavanone glycosides by β-glucosidase from Pyrococcus furiosus and its application to the production of flavanone aglycones from citrus extracts.
TLDR
The hydrolytic activity of the recombinant β-glucosidase from Pyrococcus furiosus for the flavanone glycoside hesperidin was optimal at pH 5.5 and 95 °C and the conversion yields, concentrations, and productivities in this study are the highest among those obtained from citrus extracts.
Functional and biotechnological insights into diglycosidases*
TLDR
The role of endo-β-glucosidases recognizing the heterosidic linkage in plant and filamentous fungi, as well as their prospects for technological applications are reviewed.
Enzyme‐mediated transglycosylation of rutinose (6‐O‐α‐l‐rhamnosyl‐d‐glucose) to phenolic compounds by a diglycosidase from Acremonium sp. DSM 24697
TLDR
The structure of 4‐hydroxyphenyl‐β‐rutinoside was confirmed by NMR, that is, a single glycosylated product with a free hydroxyl group was formed and is a contribution to the development of convenient and sustainable processes for the glycosolation of small phenolic compounds.
Biochemical Characterization of β-Glucosidase-mediated Catabolism of Flavonol Bisglycosides in Arabidopsis thaliana
TLDR
All biochemical data indicate that BGLU15 is essential for flavonol 3-O-glucoside and 3- o-glUCoside-7-o-rhamnoside catabolism in Arabidopsis, and is important for genetic engineering or traditional breeding strategies aimed at limiting Flavonol losses in commercially important horticultural and agricultural commodities.
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