Extracellular monoenzyme deglycosylation system of 7-O-linked flavonoid β-rutinosides and its disaccharide transglycosylation activity from Stilbella fimetaria

@article{Mazzaferro2010ExtracellularMD,
  title={Extracellular monoenzyme deglycosylation system of 7-O-linked flavonoid $\beta$-rutinosides and its disaccharide transglycosylation activity from Stilbella fimetaria},
  author={Laura S Mazzaferro and Lucrecia Pi{\~n}uel and Marisol Minig and Javier D. Breccia},
  journal={Archives of Microbiology},
  year={2010},
  volume={192},
  pages={383-393}
}
We screened for microorganisms able to use flavonoids as a carbon source; and one isolate, nominated Stilbella fimetaria SES201, was found to possess a disaccharide-specific hydrolase. It was a cell-bound ectoenzyme that was released to the medium during conidiogenesis. The enzyme was shown to cleave the flavonoid hesperidin (hesperetin 7-O-α-rhamnopyranosyl-β-glucopyranoside) into rutinose (α-rhamnopyranosyl-β-glucopyranose) and hesperetin. Since only intracellular traces of monoglycosidase… 
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References

SHOWING 1-10 OF 68 REFERENCES
Isolation and Characterization of a β-Primeverosidase-Like Enzyme from Penicillium multicolor
TLDR
It is concluded that the present enzyme is a kind of β-diglycosidase rather than β-primeverosidase, which shows narrow substrate specificity with respect to the aglycon and glycon moieties of the diglycoside.
Purification and characterization of a flavonol 3-O-β-heterodisaccharidase from the dried herb of Fagopyrum esculentum Moench
Furcatin Hydrolase from Viburnum furcatum Blume Is a Novel Disaccharide-specific Acuminosidase in Glycosyl Hydrolase Family 1*
TLDR
Phylogenetic analysis of plant β-glucosidases revealed that FH clusters with β-primeverosidase, and this suggests that the disaccharide glycosidases will form a new subfamily in glycosyl hydrolase family 1.
Purification of an isoflavonoid 7-O-β-apiosyl-glucoside β-glycosidase and its substrates from Dalbergia nigrescens Kurz
Synthesis of rutinosides and rutinose by reverse hydrolysis catalyzed by fungal α-l-rhamnosidases
The synthesis of α-l-rhamnosyl(1→6)-β-d-glucosides (rutinosides) and α-l-rhamnosyl(1→6)-β-d-glucose (rutinose) catalyzed by several fungal α-l-rhamnosidases was studied by the reverse hydrolysis…
Identification and partial characterization of glycosidic activities of commercial strains of the lactic acid bacterium, Oenococcus oeni
β-glucosidase activity was measured in cultures derived from 11 commercial preparations of Oenococcus oeni , which are utilized for the malolactic fermentation of wine. All cultures produced a…
Biocatalyzed Generation of Molecular Diversity: Selective Modification of the Saponin Asiaticoside
An array of different derivatives of the complex ursane-type triterpene glycoside asiaticoside (1), a saponin component isolated from the perennial herb Centella asiatica, was generated by exploiting…
Novel α-Glucosidase from Aspergillus nidulans with Strong Transglycosylation Activity
TLDR
It is proposed here that AgdB is a novel α-glucosidase with unusually strong transglycosylation activity and synthesized as a single polypeptide precursor.
Substrate Specificity of β-Primeverosidase, A Key Enzyme in Aroma Formation during Oolong Tea and Black Tea Manufacturing
TLDR
Results indicate the β-primeverosid enzyme, a diglycosidase, to be a key enzyme involved in aroma formation during the tea manufacturing process.
Isolation and Characterization of A β-Primeverosidase-like endo-manner β-Glycosidase from Aspergillus fumigatus AP-20
TLDR
A novel β-glycosidase-producing microorganism was isolated from soil and identified as Aspergillus fumigatus AP-20 based on its taxonomical characteristics and was purified 88-fold by fractionation with ammonium sulfate followed by successive column chromatographies on phenyl-Sepharose HP and Mono P HR.
...
1
2
3
4
5
...