Extracellular monoenzyme deglycosylation system of 7-O-linked flavonoid β-rutinosides and its disaccharide transglycosylation activity from Stilbella fimetaria
@article{Mazzaferro2010ExtracellularMD, title={Extracellular monoenzyme deglycosylation system of 7-O-linked flavonoid $\beta$-rutinosides and its disaccharide transglycosylation activity from Stilbella fimetaria}, author={Laura S Mazzaferro and Lucrecia Pi{\~n}uel and Marisol Minig and Javier D. Breccia}, journal={Archives of Microbiology}, year={2010}, volume={192}, pages={383-393} }
We screened for microorganisms able to use flavonoids as a carbon source; and one isolate, nominated Stilbella fimetaria SES201, was found to possess a disaccharide-specific hydrolase. It was a cell-bound ectoenzyme that was released to the medium during conidiogenesis. The enzyme was shown to cleave the flavonoid hesperidin (hesperetin 7-O-α-rhamnopyranosyl-β-glucopyranoside) into rutinose (α-rhamnopyranosyl-β-glucopyranose) and hesperetin. Since only intracellular traces of monoglycosidase…
30 Citations
A novel Aspergillus oryzae diglycosidase that hydrolyzes 6-O-α-L-rhamnosyl-β-D-glucoside from flavonoids
- BiologyApplied Microbiology and Biotechnology
- 2018
Abstractα-L-Rhamnosyl-β-D-glucosidase (rutinosidase) hydrolyzes the glycosidic linkage between the disaccharide 6-O-α-L-rhamnosyl-β-D-glucoside (rutinose) and the aglycone. We identified a…
Bacteria as source of diglycosidase activity: Actinoplanes missouriensis produces 6-O-α-l-rhamnosyl-β-d-glucosidase active on flavonoids
- BiologyApplied Microbiology and Biotechnology
- 2015
Findings demonstrate that organisms other than plants and filamentous fungi can contribute to an expansion of the diglycosidase toolbox.
Transglycosylation specificity of Acremonium sp. α-rhamnosyl-β-glucosidase and its application to the synthesis of the new fluorogenic substrate 4-methylumbelliferyl-rutinoside.
- Biology, ChemistryCarbohydrate research
- 2012
α-L-Rhamnosyl-β-D-glucosidase (Rutinosidase) from Aspergillus niger: Characterization and Synthetic Potential of a Novel Diglycosidase
- Biology, Chemistry
- 2015
The utility of the recombinant rutinosidase was demonstrated by its use for the synthesis of a broad spectrum of rutinoides of primary, secondary, acyclic and phenolic alcohols as well as for the preparation of free rutinose.
The flavonoid degrading fungus Acremonium sp. DSM 24697 produces two diglycosidases with different specificities
- BiologyApplied Microbiology and Biotechnology
- 2019
A phylogenetic analysis showed that αRβG I grouped in glycoside hydrolase family 5, subfamily 23 (GH5), together with other fungal diglycosidases whose substrate specificities had been reported to be different from αR βG I, and thus is the first GH3 member that hydrolyzes the heterosidic linkage of rutinosylated compounds.
α-Rhamnosyl-β-glucosidase-catalyzed reactions for analysis and biotransformations of plant-based foods.
- ChemistryJournal of agricultural and food chemistry
- 2011
The aroma precursors were significantly hydrolyzed, which indicated the potential use of the enzyme for biotechnological applications, for example, in aroma modulation of fermented foods.
Hydrolysis of flavanone glycosides by β-glucosidase from Pyrococcus furiosus and its application to the production of flavanone aglycones from citrus extracts.
- ChemistryJournal of agricultural and food chemistry
- 2013
The hydrolytic activity of the recombinant β-glucosidase from Pyrococcus furiosus for the flavanone glycoside hesperidin was optimal at pH 5.5 and 95 °C and the conversion yields, concentrations, and productivities in this study are the highest among those obtained from citrus extracts.
Functional and biotechnological insights into diglycosidases*
- Biology
- 2011
The role of endo-β-glucosidases recognizing the heterosidic linkage in plant and filamentous fungi, as well as their prospects for technological applications are reviewed.
Enzyme‐mediated transglycosylation of rutinose (6‐O‐α‐l‐rhamnosyl‐d‐glucose) to phenolic compounds by a diglycosidase from Acremonium sp. DSM 24697
- ChemistryBiotechnology and applied biochemistry
- 2019
The structure of 4‐hydroxyphenyl‐β‐rutinoside was confirmed by NMR, that is, a single glycosylated product with a free hydroxyl group was formed and is a contribution to the development of convenient and sustainable processes for the glycosolation of small phenolic compounds.
Biochemical Characterization of β-Glucosidase-mediated Catabolism of Flavonol Bisglycosides in Arabidopsis thaliana
- Biology
- 2015
All biochemical data indicate that BGLU15 is essential for flavonol 3-O-glucoside and 3- o-glUCoside-7-o-rhamnoside catabolism in Arabidopsis, and is important for genetic engineering or traditional breeding strategies aimed at limiting Flavonol losses in commercially important horticultural and agricultural commodities.
References
SHOWING 1-10 OF 68 REFERENCES
Isolation and Characterization of a β-Primeverosidase-Like Enzyme from Penicillium multicolor
- Biology, ChemistryBioscience, biotechnology, and biochemistry
- 2006
It is concluded that the present enzyme is a kind of β-diglycosidase rather than β-primeverosidase, which shows narrow substrate specificity with respect to the aglycon and glycon moieties of the diglycoside.
Furcatin Hydrolase from Viburnum furcatum Blume Is a Novel Disaccharide-specific Acuminosidase in Glycosyl Hydrolase Family 1*
- Chemistry, BiologyJournal of Biological Chemistry
- 2004
Phylogenetic analysis of plant β-glucosidases revealed that FH clusters with β-primeverosidase, and this suggests that the disaccharide glycosidases will form a new subfamily in glycosyl hydrolase family 1.
Synthesis of rutinosides and rutinose by reverse hydrolysis catalyzed by fungal α-l-rhamnosidases
- Chemistry
- 2008
The synthesis of α-l-rhamnosyl(1→6)-β-d-glucosides (rutinosides) and α-l-rhamnosyl(1→6)-β-d-glucose (rutinose) catalyzed by several fungal α-l-rhamnosidases was studied by the reverse hydrolysis…
Identification and partial characterization of glycosidic activities of commercial strains of the lactic acid bacterium, Oenococcus oeni
- Chemistry
- 2000
β-glucosidase activity was measured in cultures derived from 11 commercial preparations of Oenococcus oeni , which are utilized for the malolactic fermentation of wine. All cultures produced a…
Biocatalyzed Generation of Molecular Diversity: Selective Modification of the Saponin Asiaticoside
- Chemistry, Biology
- 2005
An array of different derivatives of the complex ursane-type triterpene glycoside asiaticoside (1), a saponin component isolated from the perennial herb Centella asiatica, was generated by exploiting…
Novel α-Glucosidase from Aspergillus nidulans with Strong Transglycosylation Activity
- Biology, ChemistryApplied and Environmental Microbiology
- 2002
It is proposed here that AgdB is a novel α-glucosidase with unusually strong transglycosylation activity and synthesized as a single polypeptide precursor.
Substrate Specificity of β-Primeverosidase, A Key Enzyme in Aroma Formation during Oolong Tea and Black Tea Manufacturing
- ChemistryBioscience, biotechnology, and biochemistry
- 2001
Results indicate the β-primeverosid enzyme, a diglycosidase, to be a key enzyme involved in aroma formation during the tea manufacturing process.
Isolation and Characterization of A β-Primeverosidase-like endo-manner β-Glycosidase from Aspergillus fumigatus AP-20
- Biology
- 2002
A novel β-glycosidase-producing microorganism was isolated from soil and identified as Aspergillus fumigatus AP-20 based on its taxonomical characteristics and was purified 88-fold by fractionation with ammonium sulfate followed by successive column chromatographies on phenyl-Sepharose HP and Mono P HR.