Extracellular metabolomics: a metabolic footprinting approach to assess fiber degradation in complex media.

@article{VillasBas2006ExtracellularMA,
  title={Extracellular metabolomics: a metabolic footprinting approach to assess fiber degradation in complex media.},
  author={Silas Granato Villas-B{\^o}as and Samantha Joan Noel and Geoffrey A. Lane and Graeme T. Attwood and Adrian L Cookson},
  journal={Analytical biochemistry},
  year={2006},
  volume={349 2},
  pages={
          297-305
        }
}
This work reports the implementation and optimization of a method for high-throughput analysis of metabolites produced by the breakdown of natural polysaccharides by microorganisms. Our simple protocol enables simultaneous separation and quantification of more than 40 different sugars and sugar derivatives, in addition to several organic acids in complex media, using 50-mul samples and a standard gas chromatography-mass spectrometry platform that was fully optimized for this purpose. As an… CONTINUE READING
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