Extracellular matrix-mediated differentiation of human embryonic stem cells: differentiation to insulin-secreting beta cells.

@article{Narayanan2014ExtracellularMD,
  title={Extracellular matrix-mediated differentiation of human embryonic stem cells: differentiation to insulin-secreting beta cells.},
  author={Karthikeyan Narayanan and Vivian Y Lim and Jiayi Shen and Zhen Wei Tan and Divya Rajendran and Shyh-Chyang Luo and Shujun Gao and Andrew C A Wan and Jackie Y. Ying},
  journal={Tissue engineering. Part A},
  year={2014},
  volume={20 1-2},
  pages={424-33}
}
Stem cells have tremendous potential for treating various human diseases. Protocols have been established to differentiate stem cells into specific lineages through the provision of signals in the form of growth factors, cytokines, or small molecules. Herein we investigate an alternative strategy for directed differentiation of human embryonic stem cells (hESCs)--extracellular-matrix (ECM) mediated differentiation. Decellularized ECM and conditioned media from the appropriate committed cell… CONTINUE READING