Extracellular matrix contraction by cultured mesangial cells: an assay system for mesangial cell-matrix interaction.

@article{Kitamura1991ExtracellularMC,
  title={Extracellular matrix contraction by cultured mesangial cells: an assay system for mesangial cell-matrix interaction.},
  author={Mineaki Kitamura and Naoki Maruyama and Haruyoshi Yoshida and Ryuji Nagasawa and Tetsuya Mitarai and Osamu Sakai},
  journal={Experimental and molecular pathology},
  year={1991},
  volume={54 3},
  pages={181-200}
}
To investigate the interaction between glomerular mesangial cells (MCs) and extracellular matrix (ECM), we have developed a quantitative assay using a floating culture of MC-populated collagen gel matrix (MCGM). Since MCs can contract such a matrix consisting of type I collagen, MCGM exhibited marked contraction. The apparent cause was tensional interaction between MCs and collagen fibers, because: (1) MCs in the gel attached tightly to surrounding collagen fibers; (2) collagen fibrils… CONTINUE READING