• DOI:10.1515/cclm.2011.881
• Corpus ID: 26045011

Extracellular matrix-associated (GAGs, CTGF), angiogenic (VEGF) and inflammatory factors (MCP-1, CD40, IFN-γ) in type 1 diabetes mellitus nephropathy

@inproceedings{Ellina2012ExtracellularM,
  title={Extracellular matrix-associated (GAGs, CTGF), angiogenic (VEGF) and inflammatory factors (MCP-1, CD40, IFN-$\gamma$) in type 1 diabetes mellitus nephropathy},
  author={Olga Ellina and Antonios Chatzigeorgiou and Sophia Kouyanou and Maria Lymberi and Christina Mylona-Karagianni and Emmanouil Tsouvalas and Elli F. Kamper},
  booktitle={Clinical chemistry and laboratory medicine},
  year={2012}
}

• Olga Ellina, A. Chatzigeorgiou, +4 authors E. Kamper
• Published in
  Clinical chemistry and…
  2012
• Medicine, Biology

Abstract Background: The aim of the present study was to evaluate the role of extracellular matrix-associated glycosaminoglycans (GAGs), connective tissue growth factor (CTGF), angiogenic vascular endothelial growth factor (VEGF) and inflammatory factors (MCP-1, CD40, IFN-γ) in the development of diabetic nephropathy in type 1 diabetes (T1DM). Methods: Plasma and urine samples from 30 T1DM patients and 20 healthy controls were used to measure the levels of CTGF, VEGF, MCP-1, CD40 and IFN-γ by ELISA. Plasma and urine GAGs were measured using a spectrophotometric method. Results: Plasma levels of GAGs, CD40 and MCP-1 and urine levels of GAGs and CTGF were significantly elevated in normoalbuminuric T1DM patients. A tendency to higher plasma VEGF levels was found in patients compared to controls. The urine/plasma GAGs ratio of T1DM patients was almost similar to that of healthy subjects (HS), whereas the urine/plasma CTGF ratio was about three times greater in diabetic patients compared to HS. Conclusions: Conclusively, increased GAGs and CTGF excretion are evident in T1DM normoalbuminuric juveniles, possibly reflecting early renal injury signs, before the initiation of albuminuria.