Extracellular matrix (ECM) proteoglycans produced by cultured bovine corneal endothelial cells.

@article{Nevo1984ExtracellularM,
  title={Extracellular matrix (ECM) proteoglycans produced by cultured bovine corneal endothelial cells.},
  author={Zvi Nevo and Roc{\'i}o Gonz{\'a}lez and Denis Gospodarowicz},
  journal={Connective tissue research},
  year={1984},
  volume={13 1},
  pages={45-57}
}
The nature of the proteoglycan(s) (PG) found in the extracellular matrix (ECM) layer produced by cultured bovine corneal endothelial (BCE) cells is analyzed. The PG(s) account for approximately 5 to 6% of the dry weight of the ECM, regardless of the amount of extracellular soluble PG available in the medium. A 4 M guanidinium chloride (GuCl) extract of ECM was separated on a dissociative cesium chloride (CsCl) gradient (1.25 g/cm3 starting density). Results showed one main peak of PG substance… CONTINUE READING

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