Extracellular hydrolysis of formyl peptides and subsequent uptake of liberated amino acids by alveolar macrophages.

  title={Extracellular hydrolysis of formyl peptides and subsequent uptake of liberated amino acids by alveolar macrophages.},
  author={J. Basilion and D. Stickle and A. Holian},
  journal={Biochimica et biophysica acta},
  volume={886 2},
The mechanism of accumulation of radioactive label from fNle-Leu-[3H]Phe by guinea pig alveolar macrophages was investigated. The binding of fNle-Leu-[3H]Phe to macrophages reached equilibrium within 5 min at 4 degrees C, but equilibrium could not be achieved at temperatures where fNle-Leu-Phe stimulated superoxide anion production is observed (e.g., 21-23 degrees C). At this temperature a rapid phase of initial binding of fNle-Leu-[3H]Phe to its receptor was followed by continued accumulation… Expand
2 Citations
Identification of a human neutrophil protein of Mr 24 000 that binds N-formyl peptides: co-sedimentation with specific granules.
The N-terminal sequence of the Mr 24 000 species was determined and it appears to be a novel protein, which will allow its relationship to the receptor, if any, to be elucidated and allow assignment of a function to this potentially important molecule. Expand
Absence of FMLP Receptors on Rat Macrophages
In 3H‐FMLP binding studies, the lack of responsiveness of peritoneal and alveolar macrophages was associated with the lackof FMLP receptors on these cell types, in striking contrast to the presence of functional receptors on rat neutrophils. Expand


Release of oxygen products from lung macrophages by N-formyl peptides.
  • A. Holian, R. Daniele
  • Chemistry, Medicine
  • Journal of applied physiology: respiratory, environmental and exercise physiology
  • 1981
Incubation of guinea pig pulmonary macrophages with N-formylmethionylphenylalanine (FMP) resulted in 1) a rapid increase in O2 consumption and 2) an accumulation of superoxide anion and hydrogenExpand
Formyl peptide stimulation of superoxide anion release from lung macrophages: Sodium and potassium involvement
A minimal direct role, if any, for K+ is suggested in the events that lead to FMP‐stimulated O  2− release by alveolar macrophages. Expand
Stimulation of oxygen consumption and superoxide anion production in pulmonary macrophages by N‐formyl methionyl peptides
The effects of FMP on oxygen consumption and superoxide anion production in guinea pig alveolar macrophages are compared to concanavalin A (Con A). Expand
Chloroquine and ammonium ion inhibit receptor-mediated endocytosis of mannose-glycoconjugates by macrophages: apparent inhibition of receptor recycling.
The results suggest that chloroquine and NH4+ inhibit receptor recycling and thereby inhibit ligand uptake in 125I-Mannose-BSA, suggesting that receptors are conserved and recycled. Expand
Immune-complex-induced transglutaminase activation: its role in the Fc-receptor-mediated transmembrane effect on peritoneal macrophages.
Methylamine, a competitive substrate inhibitor of transglutaminase could inhibit EA rosette formation as well as the soluble IC- or EA-induced lipid reordering of the plasma membrane of macrophages. Expand
Stimulation of amino acid transport into liver cells from rats adapted to a high-protein diet.
Evidence is presented suggesting that the increased uptake of amino acids by the liver of rats fed on high-protein diets is obtained by developing favourable gradients and enhancing transport capacities, where accelerated metabolism plays a decisive role. Expand
Chemotactic peptide receptor modulation in polymorphonuclear leukocytes
The binding of the FNLLP to its receptor on rabbit polymorphonuclear leukocytes modulates the number of available peptide receptors and down regulation of the chemotactic peptide receptor is concentration dependent in both the rate and extent of receptor loss. Expand
Role of secretory events in modulating human neutrophil chemotaxis.
The data indicate limited exocytosis of intracellular granule contents is associated with increased availability of PMN cehmotactic factor receptors and inactivation of chemotactic responsiveness related to increase cell adhesiveness, decreased PMN binding ofChemotactic factors, and to hydrolysis of chemoattractants by factors secreted extracellularly. Expand
Enhanced transglutaminase activity associated with macrophage activation. Possible role in Fc-mediated phagocytosis.
A possible role for TGase is suggested in macrophage activation and in receptor-dependent phagocytosis in guinea pig peritoneal macrophages. Expand
Neutrophil chemotaxis and enzyme release competitive inhibition by a diazoacetamide pepsin inhibitor.
The present data suggest that diazoacetylnorleucine methyl ester plus copper reacts with the neutrophil receptor for pepstatin and formylmethionyl peptides, and thus may be useful in further characterization of this structure. Expand