Extracellular fluid proteins of goldfish brain: evidence for the presence of proteases and esterases.

@article{Shashoua1986ExtracellularFP,
  title={Extracellular fluid proteins of goldfish brain: evidence for the presence of proteases and esterases.},
  author={Victor E. Shashoua and Barton Holmquist},
  journal={Journal of neurochemistry},
  year={1986},
  volume={47 3},
  pages={738-43}
}
Preparations of enriched fractions of extracellular fluid (ECF) proteins from goldfish brain were found to contain protease(s) and esterase(s). The N-substituted furanacryloyl (FA) peptides FA-Phe-Gly-Gly and FA-Phe-OMe were used as model substrates for determining protease and esterase activity, respectively, in a spectrophotometric assay. Studies of the profile of substrate specificity and identification of the types of compounds that were effective as inhibitors showed that these ECF enzymes… CONTINUE READING