Extracellular depolymerization of hyaluronic acid in cultured human skin fibroblasts.

Abstract

The chain length of [3H]hyaluronic acid synthesized by cultivating human skin fibroblasts in the presence of [3H]glucosamine was investigated. [3H]Hyaluronic acid obtained from the matrix fraction was excluded from a Sepharose CL-2B column irrespective of the incubation period, whereas that from the medium was depolymerized into a constant chain length (Mr = 40,000). The reducing and non-reducing terminals of the depolymerized hyaluronic acid were N-acetylglucosamine and glucuronic acid, respectively. Prolonged incubation produced no oligosaccharides as shown by examination of hyaluronidase digests, suggesting the presence of a novel endo-beta-N-acetylglucosaminidase in cultured human skin fibroblasts.

Cite this paper

@article{Nakamura1990ExtracellularDO, title={Extracellular depolymerization of hyaluronic acid in cultured human skin fibroblasts.}, author={Tadashi Nakamura and Keiichi Takagaki and Keishi Kubo and Akihiro Morikawa and Shin-ichi Tamura and Masahiro Endo}, journal={Biochemical and biophysical research communications}, year={1990}, volume={172 1}, pages={70-6} }