Extracellular calcium affects the membrane currents of cultured human keratinocytes

@article{Mauro1990ExtracellularCA,
  title={Extracellular calcium affects the membrane currents of cultured human keratinocytes},
  author={T. Mauro and P. Pappone and R. Isseroff},
  journal={Journal of Cellular Physiology},
  year={1990},
  volume={143}
}
Electrophysiologic properties of cultured human keratinocytes were studied using the patch voltage‐clamp technique. Undifferentiated, proliferative keratinocytes grown in low Ca2+ medium had an average resting membrane potential of −24 mV. Voltage‐clamp experiments showed that these cells had two membrane ionic currents: a large voltage‐independent leak conductance, and a smaller voltage‐dependent Cl− current that activated with depolarization. Increasing the extra‐cellular Ca2+ concentration… Expand
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