Extracellular calcium affects the membrane currents of cultured human keratinocytes
@article{Mauro1990ExtracellularCA, title={Extracellular calcium affects the membrane currents of cultured human keratinocytes}, author={Theodora M. Mauro and Pamela A. Pappone and Roslyn Rivkah Isseroff}, journal={Journal of Cellular Physiology}, year={1990}, volume={143} }
Electrophysiologic properties of cultured human keratinocytes were studied using the patch voltage‐clamp technique. Undifferentiated, proliferative keratinocytes grown in low Ca2+ medium had an average resting membrane potential of −24 mV. Voltage‐clamp experiments showed that these cells had two membrane ionic currents: a large voltage‐independent leak conductance, and a smaller voltage‐dependent Cl− current that activated with depolarization. Increasing the extra‐cellular Ca2+ concentration…
36 Citations
Electrophysiological characterization of human keratinocytes using the patch‐clamp technique
- BiologyExperimental dermatology
- 2000
In‐vitro cultured keratinocytes of the cell line HaCaT were characterized by means of the voltage‐clamp technique and it was concluded that this channel contributes to the Cl−‐conductance of the cellular membrane and is a determinant of the membrane potential of human keratinocyte cells.
Expression and biological significance of Ca2+‐activated ion channels in human keratinocytes
- BiologyFASEB journal : official publication of the Federation of American Societies for Experimental Biology
- 2001
RT‐PCR showed that HaCaT keratinocytes express two types of Ca2+‐activated Cl− channels, CaCC2 and CaCC3 (CLCA2), as well as the Ca2‐activated K+ channel hSK4, suggesting that h SK4 might play a role in linking changes in membrane potential to the biological fate of keratinocyte differentiation.
Kv7/M-type potassium channels in rat skin keratinocytes
- BiologyPflügers Archiv - European Journal of Physiology
- 2013
It is concluded that rat skin keratinocytes possess M-channels that, when activated, can modify their physiological properties, with potential significance for their sensory and other biological functions.
Properties of BKCa Channels in Oral Keratinocytes
- BiologyJournal of dental research
- 2005
The results demonstrate that the properties of BKCa channels in normal human oral keratinocytes are similar to those described in other types of cells, and caffeic acid derivatives can also stimulate BK Ca-channel activity directly.
Amiloride blocks a keratinocyte nonspecific cation channel and inhibits Ca(++)-induced keratinocyte differentiation.
- BiologyThe Journal of investigative dermatology
- 1995
Experiments suggest that this compound may inhibit keratinocyte differentiation by blocking the nonspecific cation channel.
A large conductance [Ca2+]i‐independent K+ channel expressed in HaCaT keratinocytes
- BiologyExperimental dermatology
- 2002
It is concluded that these K+ channels contribute to the resting potential of human keratinocytes which may control the Ca2+ influx and thereby their proliferation and differentiation.
Ion channels are linked to differentiation in keratinocytes
- BiologyThe Journal of Membrane Biology
- 2004
Keratinocyte single channel currents were studied using the patch-clamp technique to correlate ion channels with cell differentiation and investigate keratinocyte membrane responses to Ca2+, finding the most frequently observed channel was a 14 pS nonspecific cation channel.
Characterization of multiple P2X receptors in cultured normal human epidermal keratinocytes.
- BiologyThe Journal of investigative dermatology
- 2005
Assessment of native P2X receptors in normal human epidermal keratinocytes in NHEK using whole-cell patch clamp technique, RT-PCR, and determination of intracellular Ca(2+) concentration found that the expression of multiple P1-gated ion channels receptor subtype mRNA was increased and downregulated in differentiated cells.
Potassium channels and regulation of proliferation of human melanoma cells.
- BiologyThe Journal of physiology
- 1992
It is concluded that delayed rectifier potassium channels are involved in the control of melanoma cell proliferation and may be involved in controlling the driving force for a calcium influx thereby interacting with Ca(2+)‐dependent cell cycle control proteins.
A volume-sensitive chloride conductance revealed in cultured human keratinocytes by 36Cl- efflux and whole-cell patch clamp recording.
- BiologyBiochimica et biophysica acta
- 1992
References
SHOWING 1-10 OF 37 REFERENCES
Altered regulation of airway epithelial cell chloride channels in cystic fibrosis.
- Biology, MedicineScience
- 1986
The findings demonstrate the presence of chloride channels in the apical membranes of CF airway cells, which appears to be intact, but cyclic adenosine monophosphate (cAMP)-dependent control of their activity is defective.
Two types of chloride channel on duct cells cultured from human fetal pancreas.
- BiologyThe American journal of physiology
- 1989
While the physiological role of these channels is not firmly established, the small-conductance channel might function in parallel with a Cl- -HCO-3 exchanger to provide a mechanism for electrogenic bicarbonate secretion from the duct cell.
Ca2+ sensitivity of volume‐regulatory K+ and Cl‐ channels in cultured human epithelial cells.
- BiologyThe Journal of physiology
- 1988
It is concluded that both K+ and Cl‐ channels are involved in the regulatory volume decrease, and that the former channel is exclusively activated by elevation of the cytosolic Ca2+ concentration in the epithelial cells.
Calcium‐dependent chloride currents in isolated cells from rat lacrimal glands.
- BiologyThe Journal of physiology
- 1986
Isolated cells from rat lacrimal glands were studied with the tight‐seal whole‐cell recording technique and HEDTA was preferable to EGTA as a Ca2+ buffer, even under conditions of equivalent equilibrium buffering power.
Chloride channels in cultured glomus cells of the rat carotid body.
- BiologyThe American journal of physiology
- 1989
A large-conductance chloride channel is characterized with random open and closed kinetics in inside-out patches of cultured glomus cells and it is inferred that the chloride channels may play an important role in the physiology of glomUS cells by aiding in the regulation of pHi and the resting potential via bicarbonate and chloride permeability.
External calcium ions are required for potassium channel gating in squid neurons.
- BiologyScience
- 1987
Calcium is an essential cofactor in the gating of potassium channels in squid neurons and suggests that potassium channels lose their ability to close in the absence of calcium and simultaneously lose their selectivity.
Phospholipid metabolism in calcium-regulated differentiation in cultured murine keratinocytes.
- BiologyBiochemical and biophysical research communications
- 1984
Factors influencing calcium‐induced terminal differentiation in cultured mouse epidermal cells
- BiologyJournal of cellular physiology
- 1983
Mouse epidermal cells can be grown as a proliferating monolayer in medium containing 0.02‐0.1 mM calcium. Terminal differentiation of these cells with formation of cornified cells and cell death is…
Use of strontium to separate calcium‐dependent pathways for proliferation and differentiation in human keratinocytes
- BiologyJournal of cellular physiology
- 1987
The results suggest that Caex may separately stimulate keratinocyte proliferation and terminal differentiation and that Srex can substitute for Caex in the former but not the latter process.
Ionic calcium reservoirs in mammalian epidermis: ultrastructural localization by ion-capture cytochemistry.
- BiologyThe Journal of investigative dermatology
- 1985
The striking intercellular Ca++ accumulation in the mid granular layer, coupled with Ca++ influx in the upper granular layers, supports the view that changes in intracellular Ca++ may regulate epidermal differentiation and suggests that divalent cations may contribute to both lamellar body secretion and to the formation of intercorneocyte membrane bilayers.