Extracellular calcium affects the membrane currents of cultured human keratinocytes

@article{Mauro1990ExtracellularCA,
  title={Extracellular calcium affects the membrane currents of cultured human keratinocytes},
  author={Theodora M. Mauro and Pamela A. Pappone and Roslyn Rivkah Isseroff},
  journal={Journal of Cellular Physiology},
  year={1990},
  volume={143}
}
Electrophysiologic properties of cultured human keratinocytes were studied using the patch voltage‐clamp technique. Undifferentiated, proliferative keratinocytes grown in low Ca2+ medium had an average resting membrane potential of −24 mV. Voltage‐clamp experiments showed that these cells had two membrane ionic currents: a large voltage‐independent leak conductance, and a smaller voltage‐dependent Cl− current that activated with depolarization. Increasing the extra‐cellular Ca2+ concentration… 
Electrophysiological characterization of human keratinocytes using the patch‐clamp technique
TLDR
In‐vitro cultured keratinocytes of the cell line HaCaT were characterized by means of the voltage‐clamp technique and it was concluded that this channel contributes to the Cl−‐conductance of the cellular membrane and is a determinant of the membrane potential of human keratinocyte cells.
Expression and biological significance of Ca2+‐activated ion channels in human keratinocytes
  • H. Koegel, C. Alzheimer
  • Biology
    FASEB journal : official publication of the Federation of American Societies for Experimental Biology
  • 2001
TLDR
RT‐PCR showed that HaCaT keratinocytes express two types of Ca2+‐activated Cl− channels, CaCC2 and CaCC3 (CLCA2), as well as the Ca2‐activated K+ channel hSK4, suggesting that h SK4 might play a role in linking changes in membrane potential to the biological fate of keratinocyte differentiation.
Kv7/M-type potassium channels in rat skin keratinocytes
TLDR
It is concluded that rat skin keratinocytes possess M-channels that, when activated, can modify their physiological properties, with potential significance for their sensory and other biological functions.
Properties of BKCa Channels in Oral Keratinocytes
TLDR
The results demonstrate that the properties of BKCa channels in normal human oral keratinocytes are similar to those described in other types of cells, and caffeic acid derivatives can also stimulate BK Ca-channel activity directly.
Amiloride blocks a keratinocyte nonspecific cation channel and inhibits Ca(++)-induced keratinocyte differentiation.
TLDR
Experiments suggest that this compound may inhibit keratinocyte differentiation by blocking the nonspecific cation channel.
A large conductance [Ca2+]i‐independent K+ channel expressed in HaCaT keratinocytes
TLDR
It is concluded that these K+ channels contribute to the resting potential of human keratinocytes which may control the Ca2+ influx and thereby their proliferation and differentiation.
Ion channels are linked to differentiation in keratinocytes
TLDR
Keratinocyte single channel currents were studied using the patch-clamp technique to correlate ion channels with cell differentiation and investigate keratinocyte membrane responses to Ca2+, finding the most frequently observed channel was a 14 pS nonspecific cation channel.
Characterization of multiple P2X receptors in cultured normal human epidermal keratinocytes.
TLDR
Assessment of native P2X receptors in normal human epidermal keratinocytes in NHEK using whole-cell patch clamp technique, RT-PCR, and determination of intracellular Ca(2+) concentration found that the expression of multiple P1-gated ion channels receptor subtype mRNA was increased and downregulated in differentiated cells.
Potassium channels and regulation of proliferation of human melanoma cells.
TLDR
It is concluded that delayed rectifier potassium channels are involved in the control of melanoma cell proliferation and may be involved in controlling the driving force for a calcium influx thereby interacting with Ca(2+)‐dependent cell cycle control proteins.
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