Extracellular Nef protein targets CD4+ T cells for apoptosis by interacting with CXCR4 surface receptors.

@article{James2004ExtracellularNP,
  title={Extracellular Nef protein targets CD4+ T cells for apoptosis by interacting with CXCR4 surface receptors.},
  author={Cleve Orian James and Ming-Bo Huang and Mafuz Khan and Minerva Garcia-Barrio and Michael D. Powell and Vincent Bond},
  journal={Journal of virology},
  year={2004},
  volume={78 6},
  pages={3099-109}
}
The effects of soluble Nef protein on CD4(+) T cells were examined. CD4(+)-T-cell cultures exposed to soluble Nef were analyzed for apoptosis by terminal deoxynucleotidyltransferase-mediated dUTP-biotin nick end labeling and hallmarks of apoptosis including cytoplasmic shrinkage, nuclear fragmentation, DNA laddering, and caspase activation. We observed dose- and time-dependent inductions of apoptosis. DNA laddering and activated caspase 3 were also evident. Cells treated with Nef/protein kinase… CONTINUE READING

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