Extracellular Matrix-Mediated Control of Aortic Smooth Muscle Cell Growth and Migration by a Combination of Ascorbic Acid, Lysine, Proline, and Catechins

  title={Extracellular Matrix-Mediated Control of Aortic Smooth Muscle Cell Growth and Migration by a Combination of Ascorbic Acid, Lysine, Proline, and Catechins},
  author={Vadim Ivanov and Svetlana Ivanova and Mohd Waheed Roomi and Tatiana Kalinovsky and Aleksandra Niedzwiecki and Matthias Rath},
  journal={Journal of Cardiovascular Pharmacology},
Extracellular matrix (ECM) function and structure are severely compromised at atherosclerotic lesion sites, contributing to initiation and progression of the disease. This study investigated whether ECM biological properties would be beneficially affected by exposure to nutrients essential for collagen synthesis and posttranslational modification. Confluent layers of human aortic smooth muscle cells (SMC) grown on collagen substrate were cultured in the presence of the tested compounds for 7 to… 
Vitamin D modulates the composition of extracellular matrix produced by smooth muscle cells and endothelial cells from human aorta
Vitamin D is involved in a wide variety of metabolic processes, including calcium homeostasis, immune modulation, hormone secretion, cell proliferation and differentiation. Hypovitaminosis D has been
Plant-Derived Micronutrients Suppress Monocyte Adhesion to Cultured Human Aortic Endothelial Cell Layer by Modulating Its Extracellular Matrix Composition
Micronutrient-dependent reduction of monocyte adhesion to endothelium is partly mediated through specific modulation of ECM composition and properties.
The influence of ascorbic acid, TGF-beta1, and cell-mediated remodeling on the bulk mechanical properties of 3-D PEG-fibrinogen constructs.
The data show that PEG-fibrinogen hydrogels are susceptible to both hydrolysis and proteolysis, and suggest that some soluble factors may stimulate matrix remodeling by modulating SMC phenotype instead of inducing ECM synthesis in a 3-D matrix.
Inhibition of collagen synthesis by select calcium and sodium channel blockers can be mitigated by ascorbic acid and ascorbyl palmitate.
These studies confirm the adverse effects of channel blockers on collagen type l and lV deposition, the key ECM components essential for maintaining optimal structural integrity of the arterial walls and imply the benefits of ascorbate and ascorbyl palmitate supplementation in medical management of cardiovascular disease in order to compensate for adverse effects.
Quercetin and catechin synergistically inhibit angiotensin II-induced redox-dependent signalling pathways in vascular smooth muscle cells from hypertensive rats
It is demonstrated that low concentrations of singly non-effective flavonoids when are combined exert a synergistic effect in inhibiting Angiotensin II-induced redox-sensitive signalling pathways.
Research Models for Studying Vascular Calcification
In vitro cell culture models of different origins, ex vivo settings using aortic tissue and various in vivo disease-induced animal models are summarized to reflect different aspects and depict the (patho)physiologic mechanisms within the VC process.
Nutrient supplementation modulates angiotensin II-mediated atherosclerosis in ApoE KO mice.
NM consumption prior to AngII administration in ApoE KO mice minimized aortic dissections, inflammatory monocyte recruitment to plaques, plaque mass and abdominal aorta dilation, making for a more stable plaque type.
Grape Seed Extract Polyphenols Improve Resistance Artery Function in Pregnant eNOS–/– Mice
The data suggest that GSEP improve resistance artery function, potentially through antioxidant actions, and provide a basis to further investigate these beneficial effects including in the prevention of intracerebral hemorrhage.
Antioxidant Activity and Anti-wrinkle Effects of Aceriphyllum rossii Leaf Ethanol Extract
It is concluded that ARLEE has excellent antioxidant activity and even better anti-wrinkle effects than ascorbic acid in human dermal fibroblasts and could be used in functional cosmetics for the prevention or alleviation of skin wrinkles induced by ultraviolet rays.


Anti-Atherogenic Effects of a Mixture of Ascorbic Acid, Lysine, Proline, Arginine, Cysteine, and Green Tea Phenolics in Human Aortic Smooth Muscle Cells
The data suggest that the NM of ascorbic acid, tea phenolics, and selected amino acids has potential in blocking the development of atherosclerotic lesions by inhibiting atherogenic responses of vascular SMC to pathologic stimuli and warrants in vivo studies.
Ascorbate affects proliferation of guinea-pig vascular smooth muscle cells by direct and extracellular matrix-mediated effects.
The direct and matrix-mediated effects of ascorbate on the proliferation rate of vascular smooth muscle cells (VSMC) isolated from the guinea-pig aorta and its antioxidant/pro-oxidant effects and regulation of extracellular matrix composition are investigated.
Fibronectin and collagen I matrixes promote calcification of vascular cells in vitro, whereas collagen IV matrix is inhibitory.
The data suggest that matrix composition can regulate development of arterial calcification and that a subpopulation of vascular cells preferentially produces positively regulating matrix components.
Modulation of collagen synthesis by growth factors: the role of ascorbate-stimulated lipid peroxidation.
Results indicate that regulation of collagen synthesis by ascorbic acid does not occur through altering the response to EGF or Il-1, and the effects of TGF-beta and FGF on collagen synthesis appear to be sensitive to lipid peroxidation.
L-Ascorbic Acid Stimulates Expression of Smooth Muscle-Specific Markers in Smooth Muscle Cells both In Vitro and In Vivo
L-Asc and its long-lasting derivatives stimulated the production of smooth muscle-specific myosin heavy chain-1 (SM1) and calponin 1 in a dose-dependent manner in rat cultured VSMCs, and the elevated production was maintained for at least 2 weeks.
Regulation of collagen synthesis by ascorbic acid.
The results suggest that collage polypeptide synthesis, posttranslational hydroxylations, and activities of the twohydroxylases are independently regulated by ascorbate.
Subendothelial retention of lipoprotein (a). Evidence that reduced heparan sulfate promotes lipoprotein binding to subendothelial matrix.
It is hypothesized that atherogenic lipoproteins stimulate endothelial cell production of heparanase, which reduces HSPG which in turn promotes Lp(a) retention.
Type I collagen gene expression in human atherosclerosis. Localization to specific plaque regions.
Human atherosclerotic plaques exhibit nonuniform patterns of type I collagen gene expression, and it is conceivable that stimulatory/inhibitory cytokines and other factors play important roles in determining the focal nature of collagen synthesis in atherosclerosis.
Collagen types in various layers of the human aorta and their changes with the atherosclerotic process.
The types of collagen components extracted from human aortas by repeated pepsin digestion were investigated by SDS-polyacrylamide gel electrophoresis (SDS-PAGE), and type I collagen was the most predominant in the intima and adventitia but was also obviously abundant in the media.
Human atherosclerosis. I. Cell constitution and characteristics of advanced lesions of the superficial femoral artery.
This study represents a systematic analysis of the fine-structural characteristics of atherosclerotic lesions of the superficial femoral artery in man together with the growth characteristics in