Extracellular DNA can preserve the genetic signatures of present and past viral infection events in deep hypersaline anoxic basins

  title={Extracellular DNA can preserve the genetic signatures of present and past viral infection events in deep hypersaline anoxic basins},
  author={Cinzia Corinaldesi and Michael Tangherlini and Gian Marco Luna and Antonio Dell'Anno},
  journal={Proceedings of the Royal Society B: Biological Sciences},
Deep hypersaline anoxic basins (DHABs) of the Mediterranean Sea are among the most extreme ecosystems on Earth and host abundant, active and diversified prokaryotic assemblages. However, factors influencing biodiversity and ecosystem functioning are still largely unknown. We investigated, for the first time, the impact of viruses on the prokaryotic assemblages and dynamics of extracellular DNA pool in the sediments of La Medee, the largest DHAB found on Earth. We also compared, in La Medee and… 

Figures from this paper

Extracellular DNA as a genetic recorder of microbial diversity in benthic deep-sea ecosystems

The findings expand the knowledge of the contribution of extracellular microbial sequences to current estimates of prokaryotic diversity obtained through the analyses of “environmental DNA”, and open new perspectives for understanding microbial successions in benthic ecosystems.

Environmental dissolved DNA harbors meaningful biological information on microbial community structure.

Crystallizer dDNA, which accounted for lower concentrations than those previously reported for hypersaline anoxic sediments, had a mixed viral and cellular origin, was enriched in archaeal DNA and had a distinctive taxonomic composition compared to that from the cellular assemblage of the same sample.

Origin, dynamics, and implications of extracellular DNA pools in marine sediments.

Gene expression profiling of microbial activities and interactions in sediments under haloclines of E. Mediterranean deep hypersaline anoxic basins

Deep-sea hypersaline anoxic basins (DHABs) in the Eastern Mediterranean Sea are considered some of the most polyextreme habitats on Earth and sediments underlying DHAB haloclines that have cumulative physico-chemical stressors within the limits of tolerance for microoorganisms can therefore be hotspots of activity in the deep Mediterranean Sea.

Enhanced viral activity and dark CO2 fixation rates under oxygen depletion: the case study of the marine Lake Rogoznica.

It is suggested that the expansion of low-oxygen zones can trigger higher viral impacts on prokaryotic heterotrophic and chemoautotrophic metabolism, with cascading effects, neglected so far, on biogeochemical processes.

Inter-comparison of the potentially active prokaryotic communities in the halocline sediments of Mediterranean deep-sea hypersaline basins

It is suggested that, while metabolically active prokaryotic communities appear to be present in sediments underlying the three DHABs investigated, their diversity and activity are likely to be more reduced in sediment underlying the brines.

Effects of Microbial Activity and Environmental Parameters on the Degradation of Extracellular Environmental DNA from a Eutrophic Lake

The results indicate that degradation of exDNA in freshwater ecosystems is driven by the combination of both biotic and abiotic factors and it may occur very fast under particular conditions.

Diversity of marine microbes in a changing Mediterranean Sea

  • G. M. Luna
  • Environmental Science
    Rendiconti Lincei
  • 2014
This review aims at summarizing the knowledge on microbial diversity in the basin, by focusing on prokaryotes (bacteria and archaea) in pelagic and benthic habitats, byocusing on the Mediterranean Alteromonas macleodii “deep-ecotype”.

The communities and functional profiles of virioplankton along a salinity gradient in a subtropical estuary.

An Updated View of the Microbial Diversity in Deep Hypersaline Anoxic Basins

Deep hypersaline anoxic basins (DHABs) are marine extreme habitats, firstly discovered in the 1970s of the last century, located in several oceanographic regions, including the Mediterranean and Red



Viral infection plays a key role in extracellular DNA dynamics in marine anoxic systems

Overall, the data suggest that DNA released by viral lysis, because of its high lability and fast turnover, may represent an important mechanism of trophic supply for prokaryotes, particularly in systems characterized by limited availability of external Trophic sources.

Preservation, origin and genetic imprint of extracellular DNA in permanently anoxic deep‐sea sediments

The findings indicate that the extracellular DNA pool is a major archive of present/past eukaryotic gene sequences, and they highlight the importance of integrating molecular cell‐oriented approaches with molecular analyses of theextracellularDNA pool, for a better assessment of microbial diversity and temporal changes in marine benthic ecosystems.

Viruses, prokaryotes and DNA in the sediments of a deep-hypersaline anoxic basin (DHAB) of the Mediterranean Sea.

The analysis of vertical profiles of viral abundance revealed the lack of significant changes with depth in the sediment, suggesting that benthic viruses in these anoxic and hypersaline conditions are preserved or resistant to decay.

Damage and degradation rates of extracellular DNA in marine sediments: implications for the preservation of gene sequences

It is suggested that the extracellular DNA pool in marine sediments represents a repository of genetic information, which can be used for improving the understanding of the biodiversity, functioning and evolution of ecosystems over different timescales.

Simultaneous Recovery of Extracellular and Intracellular DNA Suitable for Molecular Studies from Marine Sediments

This study developed an efficient procedure to recover simultaneously DNA associated with microbial cells and extracellular DNA from the same sediment sample, and obtained evidence that extrace cellular DNA recovered from different sediments did not contain amplifiable 16S rRNA genes.

Major viral impact on the functioning of benthic deep-sea ecosystems

It is reported that viral production in deep-sea benthic ecosystems worldwide is extremely high, and that viral infections are responsible for the abatement of 80% of prokaryotic heterotrophic production.

Early diagenesis and trophic role of extracellular DNA in different benthic ecosystems

The data suggest that bioavailable extracellular DNA might represent an important nutrient source for benthic heterotrophic metabolism and open new perspectives for a better understanding of the factors that influence the functioning of benthics systems.

Quantification, base composition, and fate of extracellular DNA in marine sediments

It is calculated that more than 50% of extracellular DNA was recalcitrant to enzymatic degradation, which might explain why DNA accumulates in surface sediments and suggests that DNA might play a nonnegligible role in P biogeochemical cycle.

Protistan community patterns within the brine and halocline of deep hypersaline anoxic basins in the eastern Mediterranean Sea

The protistan communities from the basins’ brine and brine/seawater interface appear to be particularly enriched with dinoflagellates, ciliates and other alveolates, as well as fungi, and are conspicuously poor in stramenopiles.

Stratified prokaryote network in the oxic–anoxic transition of a deep-sea halocline

A 2.5-m-thick chemocline with a steep NaCl gradient at 3.3 km within the water column betweeen Bannock anoxic hypersaline brine and overlying sea water is reported, supporting some of the most biomass-rich and active microbial communities in the deep sea.