Extracellular ATP is generated by ATP synthase complex in adipocyte lipid rafts

@article{Kim2004ExtracellularAI,
  title={Extracellular ATP is generated by ATP synthase complex in adipocyte lipid rafts},
  author={Bong-Woo Kim and Hyo-Jung Choo and Jae-Ho Lee and Ji-Hyun Kim and Yeoung Gyu Ko},
  journal={Experimental & Molecular Medicine},
  year={2004},
  volume={36},
  pages={476-485}
}
Mitochondrial biogenesis is known to accompany adipogenesis to complement ATP and acetyl-CoA required for lipogenesis. Here, we demonstrated that mitochondrial proteins such as ATP synthase α and β, and cytochrome c were highly expressed during the 3T3-L1 differentiation into adipocytes. Fully-differentiated adipocytes showed a significant increase of mitochondria under electron microscopy. Analysis by immunofluorescence, cellular fractionation, and surface biotinylation demonstrated the… CONTINUE READING
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