External versus internal source of calcium during the gelation of alginate beads for DNA encapsulation.

@article{Quong1998ExternalVI,
  title={External versus internal source of calcium during the gelation of alginate beads for DNA encapsulation.},
  author={D Quong and Ronald J. Neufeld and Gudmund Skj{\aa}k-Braek and Denis Poncelet},
  journal={Biotechnology and bioengineering},
  year={1998},
  volume={57 4},
  pages={438-46}
}
Alginate gels produced by an external or internal gelation technique were studied so as to determine the optimal bead matrix within which DNA can be immobilized for in vivo application. Alginates were characterized for guluronic/mannuronic acid (G/M) content and average molecular weight using 1H-NMR and LALLS analysis, respectively. Nonhomogeneous calcium, alginate, and DNA distributions were found within gels made by the external gelation method because of the external calcium source used. In… CONTINUE READING