Extensive methylation of chloroplast DNA by a nuclear gene mutation does not affect chloroplast gene transmission in chlamydomonas.

@article{Bolen1982ExtensiveMO,
  title={Extensive methylation of chloroplast DNA by a nuclear gene mutation does not affect chloroplast gene transmission in chlamydomonas.},
  author={Paul L. Bolen and Douglas M. Grant and D R Swinton and John E. Boynton and Nicholas Wright Gillham},
  journal={Cell},
  year={1982},
  volume={28 2},
  pages={335-43}
}
Based on analysis by high pressure liquid chromatography, greater than 35% of the cytosine residues in chloroplast DNA of vegetative cells were found to be methylated constitutively in the nuclear gene mutation (me-1) of Chlamydomonas reinhardtii, which has an otherwise wild-type phenotype. Digestion of chloroplast DNA from vegetative cells and gametes of this mutant with restriction endonucleases Hpa II and Msp I reveals that in the 5'CCGG3' sequence, CpG is methylated extensively, whereas CpC… CONTINUE READING

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