Extensive alternative splicing and dual promoter usage generate Tcf-1 protein isoforms with differential transcription control properties.

@article{Wetering1996ExtensiveAS,
  title={Extensive alternative splicing and dual promoter usage generate Tcf-1 protein isoforms with differential transcription control properties.},
  author={Marc van de Wetering and J Castrop and Vladimir Korinek and Hans Clevers},
  journal={Molecular and cellular biology},
  year={1996},
  volume={16 3},
  pages={745-52}
}
Previously, we reported the isolation of cDNA clones representing four alternative splice forms of TCF-1, a T-cell-specific transcription factor. In the present study, Western blotting (immunoblotting) yielded a multitude of TCF-1 proteins ranging from 25-55 kDa, a pattern not simply explained from the known splice alternatives. Subsequent cDNA cloning, PCR… CONTINUE READING