Extensive Charge Reduction and Dissociation of Intact Protein Complexes Following Electron Transfer on a Quadrupole-Ion Mobility-Time-of-Flight MS.

@article{Lermyte2015ExtensiveCR,
  title={Extensive Charge Reduction and Dissociation of Intact Protein Complexes Following Electron Transfer on a Quadrupole-Ion Mobility-Time-of-Flight MS.},
  author={Frederik Lermyte and Jonathan P. Williams and Jeffery M Brown and Esther M. Martin and Frank Sobott},
  journal={Journal of the American Society for Mass Spectrometry},
  year={2015},
  volume={26 7},
  pages={1068-76}
}
Non-dissociative charge reduction, typically considered to be an unwanted side reaction in electron transfer dissociation (ETD) experiments, can be enhanced significantly in order to reduce the charge state of intact protein complexes to as low as 1+ on a commercially available Q-IM-TOF instrument. This allows for the detection of large complexes beyond 100,000 m/z, while at the same time generating top-down ETD fragments, which provide sequence information from surface-exposed parts of the… CONTINUE READING