Extension of the HA-detection based approach: (HCA)CON(CA)H and (HCA)NCO(CA)H experiments for the main-chain assignment of intrinsically disordered proteins.

@article{Mntylahti2011ExtensionOT,
  title={Extension of the HA-detection based approach: (HCA)CON(CA)H and (HCA)NCO(CA)H experiments for the main-chain assignment of intrinsically disordered proteins.},
  author={Sampo M{\"a}ntylahti and Maarit Hellman and Perttu Permi},
  journal={Journal of biomolecular NMR},
  year={2011},
  volume={49 2},
  pages={99-109}
}
Extensive resonance overlap exacerbates assignment of intrinsically disordered proteins (IDPs). This issue can be circumvented by utilizing (15)N, (13)C' and (1)H(N) spins, where the chemical shift dispersion is mainly dictated by the characteristics of consecutive amino acid residues. Especially (15)N and (13)C' spins offer superior chemical shift dispersion in comparison to (13)C(α) and (13)C(β) spins. However, HN-detected experiments suffer from exchange broadening of amide proton signals on… CONTINUE READING

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