Extending the scope of site-specific cysteine bioconjugation by appending a prelabeled cysteine tag to proteins using protein trans-splicing.

@article{Dhar2011ExtendingTS,
  title={Extending the scope of site-specific cysteine bioconjugation by appending a prelabeled cysteine tag to proteins using protein trans-splicing.},
  author={Tulika Dhar and Thomas Kurpiers and Henning D Mootz},
  journal={Methods in molecular biology},
  year={2011},
  volume={751},
  pages={131-42}
}
Incorporating synthetic probes site-specifically into proteins is of central interest in several areas of biotechnology and protein chemistry. Bioconjugation techniques provide a simple and effective means of chemically modifying a protein. In particular, covalent chemical modifications of cysteine residues belong to one of the most important reactions due to the unique reactivity of its thiol moiety and the relatively low abundance of this amino acid in proteins. However, such types of… CONTINUE READING
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