Extending the methodology of X-ray crystallography to allow imaging of micrometre-sized non-crystalline specimens

  title={Extending the methodology of X-ray crystallography to allow imaging of micrometre-sized non-crystalline specimens},
  author={Jianwei Miao and Pambos Charalambous and Janos Kirz and David Sayre},
The contrast and penetrating power afforded by soft X-rays when they interact with matter makes this form of radiation ideal for studying micrometre-sized objects,. But although soft X-rays areuseful for probing detail too fine for visible light microscopy in specimens too thick for electron microscopy, the highest-resolution applications of X-ray imaging have been traditionally limited to crystalline samples. Here we demonstrate imaging (at ∼75 nm resolution) of a non-crystalline sample… Expand
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Biological imaging by soft x-ray diffraction microscopy
  • D. Shapiro, P. Thibault, +8 authors D. Sayre
  • Chemistry, Materials Science
  • Proceedings of the National Academy of Sciences of the United States of America
  • 2005
This work represents a previously uncharacterized application of x-ray diffraction microscopy to a specimen of this complexity and provides confidence in the feasibility of the ultimate goal of imaging biological specimens at 10-nm resolution in three dimensions. Expand
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X-ray microscopy.
  • D. Sayre, H. Chapman
  • Chemistry, Medicine
  • Acta crystallographica. Section A, Foundations of crystallography
  • 1995
X-rays, which have played the leading role in imaging crystallizable materials, may also prove to be highly valuable in the imaging of very large non-crystalline structures. Expand
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