Extending the Limits of Quantitative Proteome Profiling with Data-Independent Acquisition and Application to Acetaminophen-Treated Three-Dimensional Liver Microtissues*

@inproceedings{Bruderer2015ExtendingTL,
  title={Extending the Limits of Quantitative Proteome Profiling with Data-Independent Acquisition and Application to Acetaminophen-Treated Three-Dimensional Liver Microtissues*
},
  author={Roland Bruderer and Oliver Martin Bernhardt and Tejas Gandhi and Sa{\vs}a M. Miladinovi{\'c} and Lin-Yang Cheng and Simon Messner and Tobias Ehrenberger and Vito Zanotelli and Yulia Butscheid and Claudia Escher and Olga Vitek and Oliver Rinner and Lukas Reiter},
  booktitle={Molecular & cellular proteomics : MCP},
  year={2015}
}
The data-independent acquisition (DIA) approach has recently been introduced as a novel mass spectrometric method that promises to combine the high content aspect of shotgun proteomics with the reproducibility and precision of selected reaction monitoring. Here, we evaluate, whether SWATH-MS type DIA effectively translates into a better protein profiling as compared with the established shotgun proteomics. We implemented a novel DIA method on the widely used Orbitrap platform and used retention… CONTINUE READING