Extending Förster resonance energy transfer measurements beyond 100 Å using common organic fluorophores: enhanced transfer in the presence of multiple acceptors.

@article{Maliwal2012ExtendingFR,
  title={Extending F{\"o}rster resonance energy transfer measurements beyond 100 {\AA} using common organic fluorophores: enhanced transfer in the presence of multiple acceptors.},
  author={Badri P. Maliwal and Sangram Raut and Rafal Fudala and S. D'Auria and Vincenzo Manuel Marzullo and Alberto Luini and Ignacy Gryczyński and Zygmunt Gryczynski},
  journal={Journal of biomedical optics},
  year={2012},
  volume={17 1},
  pages={
          011006
        }
}
Using commercially available organic fluorophores, the current applications of Förster (fluorescence) resonance energy transfer (FRET) are limited to about 80 Å. However, many essential activities in cells are spatially and/or temporally dependent on the assembly/disassembly of transient complexes consisting of large-size macromolecules that are frequently separated by distances greater than 100 Å. Expanding the accessible range for FRET to 150 Å would open up many cellular interactions to… CONTINUE READING

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