Expression vectors for Acinetobacter baylyi ADP1.

  title={Expression vectors for Acinetobacter baylyi ADP1.},
  author={Charles Daniel Murin and Kristy M. Segal and Anton V. Bryksin and Ichiro Matsumura},
  journal={Applied and environmental microbiology},
  volume={78 1},
Acinetobacter baylyi ADP1 is naturally competent and proficient at homologous recombination, so it can be transformed without restriction digests or ligation reactions. Expression vectors for this system, however, are not yet widely available. Here we describe the construction and characterization of inducible expression vectors that replicate as plasmids in A. baylyi or integrate into a nonessential part of its chromosome. These tools will facilitate the engineering of genes and genomes in… CONTINUE READING

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