Expression studies with the bidirectional pcbAB-pcbC promoter region from Acremonium chrysogenum using reporter gene fusions

@article{Menne1994ExpressionSW,
  title={Expression studies with the bidirectional pcbAB-pcbC promoter region from Acremonium chrysogenum using reporter gene fusions},
  author={Stephan Menne and Markus Walz and Ulrich K{\"u}ck},
  journal={Applied Microbiology and Biotechnology},
  year={1994},
  volume={42},
  pages={57-66}
}
Two cephalosporin genes from Acremonium chrysogenum, pcbAB and pcbC encode the ACV (α-aminoadipyl-cysteinyl-valine) synthetase and isopenicillin N-synthetase, respectively. The two adjacent genes are orientated in opposite directions on the chromosomal DNA, separated by a 1.2-kb non-translated sequence, carrying the putative promoter sequences. Complete sequencing of this intergenic region revealed differences from homologous sequences from other strains. To assess the putative promoter… CONTINUE READING

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