Expression studies and functional characterization of renal human organic anion transporter 1 isoforms.

@article{Bahn2004ExpressionSA,
  title={Expression studies and functional characterization of renal human organic anion transporter 1 isoforms.},
  author={Andrew Bahn Bahn and C Ebbinghaus and Diana Ebbinghaus and Evgeni G. Ponimaskin and Laszlo Fuzes{\"i} and Gerhard Burckhardt and Yohannes Hagos},
  journal={Drug metabolism and disposition: the biological fate of chemicals},
  year={2004},
  volume={32 4},
  pages={
          424-30
        }
}
The human organic anion transporter 1 (hOAT1) facilitates the basolateral entry of organic anions such as endogenous metabolites, xenobiotics, and drugs into the proximal tubule cells. In the present study we investigated the general occurrence of hOAT1 isoforms in the kidneys and performed functional characterizations. Kidney specimens of 10 patients were analyzed by reverse transcription-polymerase chain reaction. We detected hOAT1-2 as the main transcript in almost all patients, and weak… 
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The importance of charged amino acids in the human Organic Anion Transporter 1
TLDR
It is concluded that R466 influences the binding of glutarate, but not interaction with PAH, and R466 interacts with chloride which is a major determinant in substrate movements through the transporter.
In vitro and in vivo evidence of the importance of organic anion transporters (OATs) in drug therapy.
TLDR
For all transporters, information on cloning, tissue distribution, factors influencing OAT abundance, interaction with endogenous compounds and different drug classes, drug/drug interactions and, if known, single nucleotide polymorphisms is provided.
Decreased Renal Organic Anion Secretion and Plasma Accumulation of Endogenous Organic Anions in OAT1 Knock-out Mice*
TLDR
A critical role for OAT1 is found in the functioning of the classical pathway, and the levels of ∼60 endogenous organic anions in the plasma and urine of wild-type and knock-out mice are determined, leading to identification of several compounds with significantly higher plasma concentrations and/or lower urinary concentrations in knock- out mice, suggesting the involvement of Oat1 in their renal secretion.
Organic Anion Transporter 2 (SLC22A7) Is a Facilitative Transporter of cGMP
TLDR
It is suggested that hOAT2 represents a potential new drug target for regulating cGMP levels, and is a bidirectional facilitative transporter that can control both intracellular and extracellular levels of cG MP.
The Chloride Dependence of the Human Organic Anion Transporter 1 (hOAT1) Is Blunted by Mutation of a Single Amino Acid*
TLDR
It is concluded that Arg466 influences the binding of glutarate, but not interaction with PAH, and interacts with chloride, which is a major determinant in substrate translocation.
Putative Transmembrane Domain 12 of the Human Organic Anion Transporter hOAT1 Determines Transporter Stability and Maturation Efficiency
TLDR
These results are the first to highlight the central role of transmembrane segment (TM) 12 in maintaining the stability and in promoting the maturation efficiency of hOAT1.
The multispecific organic anion transporter family: properties and pharmacological significance.
Murine renal organic anion transporters mOAT1 and mOAT3 facilitate the transport of neuroactive tryptophan metabolites.
TLDR
Six tryptophan metabolites, including the bioactive substances KYNA, XA, and the serotonin metabolite 5-hydroxyindol acetate inhibited [(3)H]p-aminohippurate (PAH) or 6-carboxyfluorescein (6-CF) uptake by 50-85%, demonstrating that these compounds interact with OAT1 as well as with Oat3.
Drug transport by Organic Anion Transporters (OATs).
Organic Anion Transporters of the SLC22 Family: Biopharmaceutical, Physiological, and Pathological Roles
TLDR
The affinities of human OATs for drug classes and their putative importance for renal drug excretion are reported and the possible impact of single nucleotide polymorphisms are dealt with.
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