Expression, purification, and contaminant detection for structural studies of Ralstonia metallidurance ClC protein rm1

Abstract

Single-particle electron cryo-microscopy (cryo-EM) has become a popular method for high-resolution study of the structural and functional properties of proteins. However, sufficient expression and purification of membrane proteins holds many challenges. We describe methods to overcome these obstacles using ClC-rm1, a prokaryotic chloride channel (ClC… (More)
DOI: 10.1371/journal.pone.0180163

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Cite this paper

@inproceedings{Abeyrathne2017ExpressionPA, title={Expression, purification, and contaminant detection for structural studies of Ralstonia metallidurance ClC protein rm1}, author={Priyanka D. Abeyrathne and Nikolaus Grigorieff}, booktitle={PloS one}, year={2017} }