Expression profiling via novel multiplex assay allows rapid assessment of gene regulation in defined signalling pathways.

@article{Eldering2003ExpressionPV,
  title={Expression profiling via novel multiplex assay allows rapid assessment of gene regulation in defined signalling pathways.},
  author={Eric Eldering and C. Arnold Spek and Hella L. Aberson and Annette Grummels and Ingrid A. M. Derks and Alex F. de Vos and Cathal J. McElgunn and Jan Schouten},
  journal={Nucleic acids research},
  year={2003},
  volume={31 23},
  pages={e153}
}
The current interest in expression of groups of functionally related genes creates a demand for novel experimental tools. We describe a multiplex ligation-dependent amplification procedure (RT-MLPA), which accurately quantifies up to 45 transcripts of interest in a one-tube assay. The output, a set of fluorescent DNA fragments, is analysed via capillary sequencer and spreadsheet software. The procedure is highly sensitive and reproducible over a 100-fold range of input RNA, with excellent… CONTINUE READING

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