Expression profiling of the bottom fermenting yeast Saccharomyces pastorianus orthologous genes using oligonucleotide microarrays

  title={Expression profiling of the bottom fermenting yeast Saccharomyces pastorianus orthologous genes using oligonucleotide microarrays},
  author={Toshiko Minato and Satoshi Yoshida and Tatsuji Ishiguro and Emiko Shimada and Satoru Mizutani and Osamu Kobayashi and Hiroyuki Yoshimoto},
The bottom fermenting yeast Saccharomyces pastorianus is reported to have arisen as a natural hybrid of two yeast strains, S. cerevisiae and S. bayanus. The S. pastorianus genome includes S. cerevisiae‐type (Sc‐type) genes and orthologous lager‐fermenting‐yeast specific‐type (Lg‐type) genes derived from S. cerevisiae and S. bayanus, respectively. To gain insights into the physiological properties of S. pastorianus, we developed an in situ synthesized 60‐mer oligonucleotide microarray for gene… 
Variation in α‐acetolactate production within the hybrid lager yeast group Saccharomyces pastorianus and affirmation of the central role of the ILV6 gene
The results confirm the role of the Ilv6 subunit in controlling α‐acetolactate/diacetyl concentration and indicate no functional divergence between the two forms of Ilv8 and the greater contribution of the S. cerevisiae ILV6 to acetolactates production in natural brewing yeast hybrids appears rather to be due to higher levels of transcription relative to theS.
Chapter 6: The genomes of lager yeasts.
  • U. Bond
  • Biology
    Advances in applied microbiology
  • 2009
Microarray studies on lager brewer's yeasts reveal cell status in the process of autolysis.
Analysis of gene expression in DNA microarray studies on lager brewer's yeast Saccharomyces pastorianus showed that the strain which autolyzes slowly had better cell vitality despite the same autolysis stage, indicating that the global understanding ofAutolysis in yeast might be promoted.
Novel brewing yeast hybrids: creation and application
The natural interspecies Saccharomyces cerevisiae × Saccharomyces eubayanus hybrid yeast is responsible for global lager beer production and is one of the most important industrial microorganisms.
Lager Yeast Comes of Age
A network evolution within Saccharomyces species is proposed, which recognizes the ability of closely related species to exchange DNA and thus drain from a combined gene pool rather than be limited to a gene pool restricted by speciation.
Functional analysis and transcriptional regulation of two orthologs of ARO10, encoding broad-substrate-specificity 2-oxo-acid decarboxylases, in the brewing yeast Saccharomyces pastorianus CBS1483.
In insights into flavour formation in S. pastorianus, a 2-oxo-acid decarboxylase involved in production of higher alcohols is provided and 'trans' regulation across the subgenomes is revealed.


Identification of bottom‐fermenting yeast genes expressed during lager beer fermentation
It has been proposed that bottom‐fermenting yeast strains of Saccharomyces pastorianus possess at least two types of genomes, and genes of one genome have been found to be highly homologous to S. cerevisiae S288C sequences, while those of the other [Lager (Lg)‐type] are less so.
Co‐existence of two types of chromosome in the bottom fermenting yeast, Saccharomyces pastorianus
Two types of chromosome co‐exist independently in bottom fermenting yeast: one set which originated from S. bayanus and another set from S.
Chromosomal structures of bottom fermenting yeasts.
Differential transcriptional regulation of sulfur assimilation gene homologues in the Saccharomyces carlsbergensis yeast species hybrid.
It is found that both homologues of the sulfur assimilation genes MET14 and MET2 are regulated in the same pathway-specific manner, but surprisingly, with different kinetics, as the genes derived from one of the parent species are alleviated from repression much faster than the genes from the other parent.
Molecular cloning, sequence analysis, and expression of the yeast alcohol acetyltransferase gene
The ATF1 gene, which encodes alcohol acetyltransferase (AATase), was cloned from Saccharomyces cerevisiae and brewery lager yeast and the results of a hydrophobicity analysis suggested that this gene product does not have a membrane-spanning region that is significantly hydrophobic.
The dynamics of the Saccharomyces carlsbergensis brewing yeast transcriptome during a production-scale lager beer fermentation.
The transcriptome of a lager brewing yeast (Saccharomyces carlsbergensis, syn. of S. pastorianus), was analysed at 12 different time points spanning a production-scale lager beer fermentation and the regional distribution of various expression levels on the chromosomes appeared to be random or near-random.
Isolation and Characterization of a Gene Specific to Lager Brewing Yeast That Encodes a Branched-Chain Amino Acid Permease
The finding of two types of branched-chain amino acid permease gene (BAP2) in the lager brewing yeast substantiates the notion that lager brewers' yeast is a hybrid of S. cerevisiae and S. bayanus and suggests that the transcription of Lg-B AP2 is regulated differently from that of cer-BAP1 in lager Brewing yeasts.
Genomic expression programs in the response of yeast cells to environmental changes.
Analysis of genomic expression patterns in the yeast Saccharomyces cerevisiae implicated the transcription factors Yap1p, as well as Msn2p and Msn4p, in mediating specific features of the transcriptional response, while the identification of novel sequence elements provided clues to novel regulators.