Expression profiling of purified mouse gonadal somatic cells during the critical time window of sex determination reveals novel candidate genes for human sexual dysgenesis syndromes.
@article{Beverdam2006ExpressionPO, title={Expression profiling of purified mouse gonadal somatic cells during the critical time window of sex determination reveals novel candidate genes for human sexual dysgenesis syndromes.}, author={Annemiek Beverdam and Peter Koopman}, journal={Human molecular genetics}, year={2006}, volume={15 3}, pages={ 417-31 } }
Despite the identification of SRY as the testis-determining gene in mammals, the genetic interactions controlling the earliest steps of male sex determination remain poorly understood. In particular, the molecular lesions underlying a high proportion of human XY gonadal dysgenesis, XX maleness and XX true hermaphroditism remain undiscovered. A number of screens have identified candidate genes whose expression is modulated during testis or ovary differentiation in mice, but these screens have…
Figures and Tables from this paper
157 Citations
New Candidate Genes Identified for Controlling Mouse Gonadal Sex Determination and the Early Stages of Granulosa and Sertoli Cell Differentiation1
- BiologyBiology of reproduction
- 2010
The development of a unique mouse carrying two transgenes that allow XX and XY mice to be distinguished as early as Embryonic Day 11.5 and previously unidentified sexually dimorphic transcripts, including low-level expressed genes such as Sry, a gene not identified in other microarray studies are reported.
Purification and Transcriptomic Analysis of Mouse Fetal Leydig Cells Reveals Candidate Genes for Specification of Gonadal Steroidogenic Cells1
- BiologyBiology of reproduction
- 2015
This dataset provides a platform for exploring the biology of FLCs and understanding the role of these cells in testicular development and provides a basis for targeted studies designed to identify causes of idiopathic XY DSD.
Identification of candidate gonadal sex differentiation genes in the chicken embryo using RNA-seq
- BiologyBMC genomics
- 2015
Insight is provided into the earliest steps of vertebrate gonad sex differentiation, and novel candidate genes for ovarian and testicular development are identified that play an important role in this tissue.
The Maestro (Mro) Gene Is Dispensable for Normal Sexual Development and Fertility in Mice
- BiologyPloS one
- 2008
It is concluded that Maestro is dispensable for normal male sexual development and fertility in laboratory mice; however, the Mro locus itself does have utility as a site for insertion of transgenes for future studies in the fields ofSexual development and Sertoli cell function.
Identification of Novel Markers of Mouse Fetal Ovary Development
- BiologyPloS one
- 2012
The data expands the set of markers of early mouse ovary differentiation and identifies a classification of early ovarian genes, thus providing additional avenues with which to dissect this process.
Sex-specific expression of a novel gene Tmem184a during mouse testis differentiation.
- BiologyReproduction
- 2007
It is shown that Tmem184a is expressed at high levels in the developing testis from 11.5 dpc, a time of active proliferation and differentiation, suggesting that it may mediate sex-specific signaling events during Sertoli cell differentiation.
Transcriptional profile of mouse pre-granulosa and Sertoli cells isolated from early-differentiated fetal gonads.
- BiologyGene expression patterns : GEP
- 2007
Purified pre-sertoli cells express genes involved in cell proliferation and cell signalling during a critical window in male sex determination
- Biology
- 2007
These data represent the earliest comprehensive microarray expression analysis of purified presumptive pre Sertoli cells available to date and coded notably for celi signalling and extra cellular proteins.
Global Survey of Protein Expression during Gonadal Sex Determination in Mice*
- BiologyMolecular & Cellular Proteomics
- 2009
The first global survey of the mouse gonad proteome at the time of sex determination by using two-dimensional nanoflow LC-MS/MS revealed potential novel regulators of gonad development and sex determination not revealed previously by transcriptomics and proteomics studies and more than 60 proteins with potential links to human disorders of sexual development.
Regulation of the gonadal transcriptome during sex determination and testis morphogenesis: comparative candidate genes.
- BiologyReproduction
- 2007
The genes and gene network identified influence numerous cellular processes with cellular differentiation, proliferation, focal contact, RNA localization, and development being predominant.
65 References
Gene expression during sex determination reveals a robust female genetic program at the onset of ovarian development.
- BiologyDevelopmental biology
- 2005
Pre-Sertoli Specific Gene Expression Profiling Reveals Differential Expression of Ppt1 and Brd3 Genes Within the Mouse Genital Ridge at the Time of Sex Determination1
- BiologyBiology of reproduction
- 2004
It is concluded that combining transgenically marked fluorescent cell populations with differential expression screening is useful for cell expression profiling in developmental systems such as sex determination and differentiation.
Sexually dimorphic gene expression in the developing mouse gonad.
- BiologyGene expression patterns : GEP
- 2002
Sexually dimorphic expression of protease nexin-1 and vanin-1 in the developing mouse gonad prior to overt differentiation suggests a role in mammalian sexual development.
- BiologyHuman molecular genetics
- 2000
In situ hybridization analysis is shown that two genes identified by this screen, protease nexin-1 (Pn-1) and vanin- 1 (Vnn1), exhibit male-specific expression prior to overt gonadal differentiation and are detected in the somatic portion of the developing gonad, suggesting a possible direct link to the testis-determining pathway for both genes.
A male-specific role for SOX9 in vertebrate sex determination.
- BiologyDevelopment
- 1996
The conservation of sexually dimorphic expression in two vertebrate classes which have significant differences in their sex determination mechanisms, points to a fundamental role for SOX9 in testis determination in vertebrates.
Subtractive hybridisation screen identifies sexually dimorphic gene expression in the embryonic mouse gonad
- BiologyGenesis
- 2003
One novel gene may be a candidate for interactions with WT1, based on its localisation to Sertoli cells and map position (16q24.3), and two genes that showed higher expression in the testis than the ovary are identified.
Profiling Gene Expression During the Differentiation and Development of the Murine Embryonic Gonad1
- BiologyBiology of reproduction
- 2005
A large number of transcripts comprising the murine transcriptome can be examined throughout male and female embryonic gonad development and allow for a more complete description of gonad differentiation and development.
Large-scale screen for genes involved in gonad development
- BiologyMechanisms of Development
- 2000
Mouse Dax1 expression is consistent with a role in sex determination as well as in adrenal and hypothalamus function
- Biology, MedicineNature Genetics
- 1996
The isolation of the mouse Dax1 gene and its pattern of expression during development suggest a basis for adrenal insufficiency and hypogonadotropic hypog onadotropicism in males affected by adrenal hypoplasia congenita and are consistent with a role for DAX1 in gonadal sex determination.
Mammalian sex determination: from gonads to brain.
- BiologyMolecular genetics and metabolism
- 1998
A regulatory cascade hypothesis for mammalian sex determination, proposing that SRY represses a negative regulator of male development, was recently supported by observation of mice that expressed a DAX1 transgene and developed as XY sex-reversed females.