Expression profiling and identification of novel genes involved in myogenic differentiation

@article{Tomczak2004ExpressionPA,
  title={Expression profiling and identification of novel genes involved in myogenic differentiation},
  author={Kinga K. Tomczak and Voichita D. Marinescu and Marco Ramoni and Despina Sanoudou and Federica Montanaro and Mei Han and Louis M. Kunkel and Isaac S. Kohane and Alan H. Beggs},
  journal={The FASEB Journal},
  year={2004},
  volume={18}
}
Skeletal muscle differentiation is a complex, highly coordinated process that relies on precise temporal gene expression patterns. To better understand this cascade of transcriptional events, we used expression profiling to analyze gene expression in a 12‐day time course of differentiating C2C12 myoblasts. Cluster analysis specific for time‐ordered microarray experiments classified 2895 genes and ESTs with variable expression levels between proliferating and differentiating cells into 22… 
A Functional Genomics Approach for Characterizing the Role of Six Transcription Factors in Muscle Development
TLDR
Results show that Six1 and Six4 can both independently regulate different genes, but can also cooperate together with other TFs where they play an important role in the proper regulation of skeletal muscle development.
Analysis of early C2C12 myogenesis identifies stably and differentially expressed transcriptional regulators whose knock-down inhibits myoblast differentiation.
TLDR
It is shown that several TRs critical for C2C12 myogenesis are not differentially regulated, suggesting that approaches that focus functional studies on differentially-expressed transcripts will fail to provide a comprehensive view of this complex process.
Tissue-specific transcript annotation and expression profiling with complementary next-generation sequencing technologies
TLDR
Next-generation sequencing of CAGE and SAGE libraries provides consistent expression levels and can enrich current genome annotations with tissue-specific promoters and alternative 3′-UTR usage and provide more insight into the transcriptional control of myogenesis.
Expression and subcellular localization of myogenic regulatory factors during the differentiation of skeletal muscle C2C12 myoblasts
TLDR
The results exhibit that MRFs nucleo‐cytoplasmic trafficking is involved in muscle differentiation and suggest that, besides the MRFs expression level, also MRFs subcellular localization, related to their functional activity, plays a key role as a regulatory step in transcriptional control mechanisms.
Network Analysis for the Identification of Differentially Expressed Hub Genes Using Myogenin Knock-down Muscle Satellite Cells
TLDR
It is proposed that MYOG exerts its regulatory effects by acting upstream of CTNNA2, which in turn regulates the differentiation of C2C12 cells via interaction with ECM genes.
Large-scale gene expression analysis of human skeletal myoblast differentiation
Glycogenome expression dynamics during mouse C2C12 myoblast differentiation suggests a sequential reorganization of membrane glycoconjugates
TLDR
A broad description of the expression dynamics of glycogenes during C2C12 differentiation is provided, providing a clearer indication of how the plasma membrane and extracellular matrix may be modified prior to cell fusion.
Identification of Genes Differentially Expressed in Myogenin Knock-Down Bovine Muscle Satellite Cells during Differentiation through RNA Sequencing Analysis
TLDR
The results of the present study widen the knowledge of the molecular basis of skeletal muscle development and reveal the vital regulatory role of MyoG in retaining muscle cell differentiation in primary bovine MSCs.
...
...

References

SHOWING 1-10 OF 54 REFERENCES
Dynamic gene expression during the onset of myoblast differentiation in vitro.
Gene expression changes during mouse skeletal myoblast differentiation revealed by transcriptional profiling.
TLDR
High-density oligonucleotide arrays are utilized to characterize changes in global mRNA expression patterns during proliferation, cell cycle withdrawal, and terminal differentiation in mouse C2C12 myoblasts to provide new insight into how genes involved in these cellular processes may play a role in skeletal muscle growth and differentiation.
Genome‐wide examination of myoblast cell cycle withdrawal during differentiation
TLDR
Because C2C12 myoblasts withdraw from the cell cycle during myocyte differentiation following a course that recapitulates this process in vivo, a genome‐wide screen revealed groups of gene products involved in cell cycle withdrawal, muscle differentiation, and apoptosis.
Calcineurin initiates skeletal muscle differentiation by activating MEF2 and MyoD.
TLDR
It is demonstrated that calcineurin regulates skeletal muscle differentiation by activating MEF2 and MyoD transcription factors leading to the induction of myogenin expression.
Expression profiling reveals altered satellite cell numbers and glycolytic enzyme transcription in nemaline myopathy muscle
TLDR
This comprehensive study of downstream molecular consequences of NM gene mutations provides insights in the cellular events leading to the NM phenotype, and transcript level changes of multiple genes suggest possible changes in Ca2+ homeostasis.
Myogenin expression, cell cycle withdrawal, and phenotypic differentiation are temporally separable events that precede cell fusion upon myogenesis
TLDR
Despite the asynchrony in the commitment to differentiation, skeletal myogenesis is a highly ordered process of temporally separable events that begins with myogenin expression, followed by p21 induction and cell cycle arrest, then phenotypic differentiation, and finally, cell fusion.
Molecular mechanisms regulating myogenic determination and differentiation.
TLDR
Recent experiments examining the origin and stem-cell capacity of satellite cells suggest that these cells may originate from the vascular system, are multipotential and may be useful for the treatment of several degenerative diseases.
The molecular regulation of myogenesis
TLDR
A functional role for MyoD during satellite cell activation and muscle repair has been identified in vivo, which cannot be substituted for by the other MRFs, putting forward the hypothesis that these factors also play specific biological roles following muscle injury and repair.
p21(CIP1) and p57(KIP2) control muscle differentiation at the myogenin step.
TLDR
It is shown that two Cdk inhibitors, p21(CIP1) and p57(KIP2), redundantly control differentiation of skeletal muscle and alveoli in the lungs and indicates a role for cell-cycle exit in myogenin function.
Molecular mechanisms regulating myogenic determination and differentiation.
TLDR
Recent experiments examining the origin and stem-cell capacity of satellite cells suggest that these cells may originate from the vascular system, are multipotential and may be useful for the treatment of several degenerative diseases.
...
...