Expression of wild-type and mutant medium-chain acyl-CoA dehydrogenase (MCAD) cDNA in eucaryotic cells.

@article{Jensen1992ExpressionOW,
  title={Expression of wild-type and mutant medium-chain acyl-CoA dehydrogenase (MCAD) cDNA in eucaryotic cells.},
  author={Tina Gudrun Jensen and Brage S Andresen and Peter Alexander. Bross and Uffe Birk Jensen and Elisabeth Holme and Steen K{\o}lvraa and N. Gregersen and Lars Bolund},
  journal={Biochimica et biophysica acta},
  year={1992},
  volume={1180 1},
  pages={
          65-72
        }
}
An effective EBV-based expression system for eucaryotic cells has been developed and used for the study of the mitochondrial enzyme medium-chain acyl-CoA dehydrogenase (MCAD). 1325 bp of PCR-generated MCAD cDNA, containing the entire coding region, was placed between the SV40 early promoter and polyadenylation signals in the EBV-based vector. Both wild-type MCAD cDNA and cDNA containing the prevalent disease-causing mutation A to G at position 985 of the MCAD cDNA were tested. In transfected… CONTINUE READING
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