Expression of unmodified hepatitis C virus envelope glycoprotein-coding sequences leads to cryptic intron excision and cell surface expression of E1/E2 heterodimers comprising full-length and partially deleted E1.

@article{Dumonceaux2003ExpressionOU,
  title={Expression of unmodified hepatitis C virus envelope glycoprotein-coding sequences leads to cryptic intron excision and cell surface expression of E1/E2 heterodimers comprising full-length and partially deleted E1.},
  author={Julie Dumonceaux and Emmanuel G. Cormier and Francis Kajumo and Gerald P. Donovan and Jayanta Roy-Chowdhury and Ira J. Fox and Jason P Gardner and Tatjana Dragic},
  journal={Journal of virology},
  year={2003},
  volume={77 24},
  pages={13418-24}
}
Hepatitis C virus (HCV) is a positive-strand RNA virus that replicates exclusively in the cytoplasm of infected cells. The viral envelope glycoproteins, E1 and E2, appear to be retained in the endoplasmic reticulum, where viral budding is thought to occur. Surprisingly, we found that the expression system used to generate HCV envelope glycoproteins influences their subcellular localization and processing. These findings have important implications for optimizing novel HCV fusion and entry… CONTINUE READING