Expression of type 1 angiotensin II receptor subtypes and angiotensin II-induced calcium mobilization along the rat nephron.

@article{Bouby1997ExpressionOT,
  title={Expression of type 1 angiotensin II receptor subtypes and angiotensin II-induced calcium mobilization along the rat nephron.},
  author={N Bouby and Annette Hus-Citharel and Jeannine Marchetti and Lise Bankir and Pierre Corvol and C Llorens-cortes},
  journal={Journal of the American Society of Nephrology : JASN},
  year={1997},
  volume={8 11},
  pages={1658-67}
}
The localization of two type 1 angiotensin II receptor subtype mRNA, AT1A and AT1B, was determined by reverse transcription-PCR on microdissected glomeruli and nephron segments. The coupling sensitivity of these two receptor subtypes was evaluated by measuring variations in intracellular calcium ([Ca2+]i) elicited by angiotensin II (Ang II) in structures expressing either AT1A or AT1B mRNA, using Fura-2 fluorescence. The highest expression of AT1 mRNA was found in glomerulus, proximal tubule… CONTINUE READING