Expression of truncated Babesia gibsoni thrombospondin-related adhesive proteins in Escherichia coli and evaluation of their diagnostic potential by enzyme-linked immunosorbent assay.

@article{Narantsatsral2011ExpressionOT,
  title={Expression of truncated Babesia gibsoni thrombospondin-related adhesive proteins in Escherichia coli and evaluation of their diagnostic potential by enzyme-linked immunosorbent assay.},
  author={Sandagdorj Narantsatsral and Youn-Kyoung Goo and Badger Battsetseg and Punsantsogvoo Myagmarsuren and Mohamad Alaa Terkawi and Takehisa Soma and Yuzi Luo and Yan Li and Shinuo Cao and Longzheng Yu and Ketsarin Kamyingkird and Gabriel Oluga Aboge and Yoshifumi Nishikawa and Xuenan Xuan},
  journal={Experimental parasitology},
  year={2011},
  volume={129 2},
  pages={
          196-202
        }
}
Among the previously established enzyme-linked immunosorbent assays (ELISAs), an ELISA using the full length of a recombinant thrombospondin-related adhesive protein of Babesia gibsoni (rBgTRAPf) is considered as the most sensitive diagnostic method for the detection of an antibody to B. gibsoni in dogs. However, the expression of rBgTRAPf in high concentration is poor and, thus, limits its usefulness as a diagnostic antigen. To improve its expression level, we have truncated BgTRAPf into two… CONTINUE READING
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