Expression of trimeric CD40 ligand in Pichia pastoris: use of a rapid method to detect high-level expressing transformants.

@article{McGrew1997ExpressionOT,
  title={Expression of trimeric CD40 ligand in Pichia pastoris: use of a rapid method to detect high-level expressing transformants.},
  author={Jeffrey T McGrew and Danielle L. Leiske and Bernard Dell and Rainer Klinke and D A Krasts and S F Wee and Nathan Abbott and Richard Armitage and Kristen Harrington},
  journal={Gene},
  year={1997},
  volume={187 2},
  pages={193-200}
}
Pichia pastoris is a yeast capable of expressing large amounts of some proteins. When expression vectors are introduced into P. pastoris, individual transformants typically express widely varying amounts of protein. Because clones expressing the highest level of protein occur infrequently during the transformation process, finding them can be very labor-intensive. We developed an immunological based filter screening method that rapidly detects transformants secreting large amounts of a… CONTINUE READING

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