Expression of transfected mutant beta-actin genes: alterations of cell morphology and evidence for autoregulation in actin pools.

@article{Leavitt1987ExpressionOT,
  title={Expression of transfected mutant beta-actin genes: alterations of cell morphology and evidence for autoregulation in actin pools.},
  author={John Leavitt and Samuel Yao-Ming Ng and Ueli Aebi and Murali Varma and G. I. Latter and Stephen L. Burbeck and L. Kedes and P. Gunning},
  journal={Molecular and cellular biology},
  year={1987},
  volume={7 7},
  pages={2457-66}
}
Two different mutant human beta-actin genes have been introduced into normal diploid human (KD) fibroblasts and their immortalized derivative cell line, HuT-12, to assess the impact of an abnormal cytoskeletal protein on cellular phenotypes such as morphology, growth characteristics, and properties relating to the neoplastic phenotype. A mutant beta-actin containing a single mutation (Gly-244----Asp-244) was stable and was incorporated into cytoskeletal stress fibers. Transfected KD cells which… CONTINUE READING