Expression of transcription regulating genes in human preimplantation embryos.

@article{AbdelRahman1995ExpressionOT,
  title={Expression of transcription regulating genes in human preimplantation embryos.},
  author={B Abdel-Rahman and Morris Fiddler and Daniel Allen Rappolee and Eugene P Pergament},
  journal={Human reproduction},
  year={1995},
  volume={10 10},
  pages={2787-92}
}
Utilizing a sensitive reverse transcriptase-polymerase chain reaction (RT-PCR) assay system, the time course of mRNA expression of two transcription regulators, OCT4 and OCT6, was assessed in individual preimplantation human embryos. Examination of ova with three pronuclei and 1-cell through blastocyst stage embryos revealed that OCT4 mRNA was continuously expressed between the time of fertilization and 10+ cell stages, whereas OCT6 mRNA expression was not observed until the 10+ cell stage. The… CONTINUE READING