Expression of the tomato ringsport nepovirus movement and coat proteins in protoplasts.

@article{Sanfaon1995ExpressionOT,
  title={Expression of the tomato ringsport nepovirus movement and coat proteins in protoplasts.},
  author={H{\'e}l{\`e}ne Sanfaçon and A. Wieczorek and Fabienne Hans},
  journal={The Journal of general virology},
  year={1995},
  volume={76 ( Pt 9)},
  pages={
          2299-303
        }
}
Tomato ringspot nepovirus (TomRSV) produces a 45 kDa movement protein and a 58 kDa coat protein in infected plants. Accumulation of the movement protein in relation to that of the coat protein was studied in infected protoplasts using a monoclonal antibody against the movement protein and polyclonal antibodies against the coat protein. Unlike most other viral movement proteins, the TomRSV movement protein was present at late stages of infection. Pulse-chase labelling experiments revealed that… 
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Characterization of the membrane association of Tomato ringspot virus proteins containing the NTB binding domain
TLDR
Proteinase K protection assays using purified membranes from infected plants revealed that although the central portion of NTB is exposed to the cytoplasmic face of the membranes, an 8 kDa fragment, recognized by antiVPg antibodies, is protected by the membranes.
Subcellular Location and Expression of Tobacco Necrosis Necrovirus p7a Protein
TLDR
Differences in the detectable amounts of both proteins may reflect their differential stability in extracts from necrotic tissue and/or the transient expression of the putative movement protein early in the replication cycle of TNV-D.
Tomato Ringspot Virus Proteins Containing the Nucleoside Triphosphate Binding Domain Are Transmembrane Proteins That Associate with the Endoplasmic Reticulum and Cofractionate with Replication Complexes
TLDR
Proteinase K protection assays provide evidence that proteins containing the NTB domain are transmembrane proteins associated with ER-derived membranes and support the hypothesis that one or several of the proteinscontaining the N TB domain anchor the replication complex to the ER.
Interaction between two tomato ringspot virus replication proteins and endoplasmic reticulum membranes
TLDR
Two hydrophobic proteins encoded by T o R S V R N A 1, termed N T B V P g and X 2, were studied for their ability to associate with intracellular membranes in planta and in vitro to identify the viral membrane anchor proteins for the replication complex.
Proteolytic processing of tomato ringspot nepovirus 3C-like protease precursors: definition of the domains for the VPg, protease and putative RNA-dependent RNA polymerase.
TLDR
Direct N-terminal sequencing of the genomic RNA-linked VPg, of the mature protease purified from E. coli extracts and of radiolabelled mature polymerases purified from in vitro translation products revealed the sequences of the NTB-VPg, VPg-Pro and Pro-Pol dipeptide cleavage sites to be Q/S, Q/G and Q/s, respectively.
Cloning and sequencing of peach rosette mosaic virus RNA1.
Mutagenesis of amino acids at two tomato ringspot nepovirus cleavage sites: effect on proteolytic processing in cis and in trans by the 3C-like protease.
TLDR
Analysis of ToRSV cleavage sites identified previously and in this study revealed that cleavage occurs at conserved Q/(G or S) dipeptides, and a Cys or Val is found in the -2 position.
Recovery of Nicotiana benthamiana Plants from a Necrotic Response Induced by a Nepovirus Is Associated with RNA Silencing but Not with Reduced Virus Titer
TLDR
It is demonstrated that although RNA silencing is active during recovery, reduction of virus titer is not required for the initiation of this phenotype, and the possibility that viral proteins, viral RNAs, and/or virus-derived siRNAs inactivate host defense responses is discussed.
Characterization of the X4 protein of Tomato ringspot virus and analysis of its variability among virus isolates
TLDR
It is suggested that within the context of a youth-services agency such as this, where children with special needs are placed in the care of adults, is a more appropriate role than in the case of a private sector agency.
Symptom recovery in Tomato ringspot virus infected Nicotiana benthamiana plants : investigation into the role of plant RNA silencing mechanisms

References

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