Expression of the recombinant Klebsiella aerogenes UreF protein as a MalE fusion.

@article{Kim1999ExpressionOT,
  title={Expression of the recombinant Klebsiella aerogenes UreF protein as a MalE fusion.},
  author={Keun Yong Kim and Chun Ho Yang and Mann Hyung Lee},
  journal={Archives of pharmacal research},
  year={1999},
  volume={22 3},
  pages={274-8}
}
Expression of the active urease of the enterobacterium, Klebsiella aerogenes, requires the presence of the accessory genes (ureD, ureE, ureF, and ureG) in addition to the three structural genes (ureA, ureB, and ureC). These accessory genes are involved in functional assembly of the nickel-metallocenter for the enzyme. Characterization of ureF gene has been hindered, however, since the UreF protein is produced in only minute amount compared to other urease gene products. In order to overexpress… CONTINUE READING