Expression of the pea gene PSMTA in E. coli. Metal-binding properties of the expressed protein.

@article{Tommey1991ExpressionOT,
  title={Expression of the pea gene PSMTA in E. coli. Metal-binding properties of the expressed protein.},
  author={Andrew M. Tommey and Jishu Shi and William P. Lindsay and Peter E Urwin and Nigel J. Robinson},
  journal={FEBS letters},
  year={1991},
  volume={292 1-2},
  pages={48-52}
}
The pea (Pisum sativum L.) gene PSMTA has an ORF encoding a predicted protein with sequence similarity to class I metallothioneins (MTS). To examine the metal-binding properties of the PSMTA protein it has been expressed in E. coli as a carboxyterminal extension of glutathione-S-transferase (GST). Metal ions were associated with the expressed protein when purified from lysates of E. coli grown in metal supplemented media. The pH of half-dissociation of Zn, Cd and Cu ions from the recombinant… CONTINUE READING

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