Expression of the p 20 Gene from Bacillus thuringiensis H-14 Increases Cry 11 A Toxin Production and Enhances Mosquito-Larvicidal Activity in Recombinant Gram-Negative Bacteria

@inproceedings{XU2001ExpressionOT,
  title={Expression of the p 20 Gene from Bacillus thuringiensis H-14 Increases Cry 11 A Toxin Production and Enhances Mosquito-Larvicidal Activity in Recombinant Gram-Negative Bacteria},
  author={Y. XU and Mary Nagai and Michael Bagdasarian and Thomas W. Smith and E. D. WALKER},
  year={2001}
}
  • XU Y., Mary Nagai, +2 authors E. D. WALKER
  • Published 2001
Experimental analyses with recombinant Escherichia coli and Pseudomonas putida transformed with plasmids bearing genes coding for the Cry11A toxin and P20 protein from Bacillus thuringiensis H-14 showed that cells producing both proteins were more toxic when fed to third-instar Aedes aegypti larvae than were cells expressing cry11A alone; the 50% lethal concentrations were in the range of 10 to 10 cells/ml. Western blots revealed a higher production of Cry11A when the p20 gene was coexpressed… CONTINUE READING
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Evaluation of synergistic interactions among CryIVA, CryIVB and CryIVD toxic components of Bacillus thuringiensis subsp. israelensis crystals

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