Expression of the nucleoprotein gene of rabies virus for use as a diagnostic reagent.

@article{He2006ExpressionOT,
  title={Expression of the nucleoprotein gene of rabies virus for use as a diagnostic reagent.},
  author={Ying-hui He and Dong-mei Gao and M Zhang},
  journal={Journal of virological methods},
  year={2006},
  volume={138 1-2},
  pages={147-51}
}
The nucleoprotein (N) gene of rabies virus CTN strain, was cloned, sequenced and expressed in Escherichia coli as a fusion with maltose binding protein (MBP). The antigenicity of this recombinant MBP-N fusion protein was examined by Western blotting and enzyme linked immunosorbent assay (ELISA). Subsequently, an indirect ELISA was developed to detect rabies specific antibody levels. Using sera from naive and vaccinated animals the ELISA results were compared with virus neutralizing antibodies… CONTINUE READING