Expression of the nucleocytoplasmic tobacco lectin in the yeast Pichia pastoris.

@article{Lannoo2007ExpressionOT,
  title={Expression of the nucleocytoplasmic tobacco lectin in the yeast Pichia pastoris.},
  author={Nausica{\"a} Lannoo and Wouter Vervecken and Paul Proost and Pierre Roug{\'e} and Els J. M. van Damme},
  journal={Protein expression and purification},
  year={2007},
  volume={53 2},
  pages={275-82}
}
The Nicotiana tabacum lectin, also called Nictaba, is a nucleocytoplasmic plant lectin expressed in tobacco leaves after exposure to jasmonates. Purification of the lectin from raw material is a time-consuming process, demanding large amounts of induced plant material. In addition, the lectin yield is low and purified lectin fractions are always contaminated with low molecular weight compounds such as phenols. In a way to improve and facilitate the purification of the tobacco lectin, we cloned… CONTINUE READING

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