Expression of the human DNA glycosylase hSMUG1 in Trypanosoma brucei causes DNA damage and interferes with J biosynthesis.

@article{Ulbert2002ExpressionOT,
  title={Expression of the human DNA glycosylase hSMUG1 in Trypanosoma brucei causes DNA damage and interferes with J biosynthesis.},
  author={Sebastian Ulbert and Mike Cross and Robert J. Boorstein and George W. Teebor and Piet Borst},
  journal={Nucleic acids research},
  year={2002},
  volume={30 18},
  pages={
          3919-26
        }
}
In kinetoplastid flagellates such as Trypanosoma brucei, a small percentage of the thymine residues in the nuclear DNA is replaced by the modified base beta-D-glucosyl-hydroxymethyluracil (J), mostly in repetitive sequences like the telomeric GGGTTA repeats. In addition, traces of 5-hydroxymethyluracil (HOMeUra) are present. Previous work has suggested that J is synthesised in two steps via HOMedU as an intermediate, but as J synthesising enzymes have not yet been identified, the biosynthetic… CONTINUE READING

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