Expression of the fusion protein of dengue virus capsid protein C and staphylococcal nuclease in Escherichia coli

@inproceedings{Cheng2004ExpressionOT,
  title={Expression of the fusion protein of dengue virus capsid protein C and staphylococcal nuclease in Escherichia coli},
  author={Qin Cheng},
  year={2004}
}
Objective: To express the fusion protein of dengue vi rus capsid(DEN) C and staphylococcal nuclease in Escherichia coli. Methods: The DEN C gene was ligated with SN gene at BamHⅠ site. The fusion gene was then subcloned into the pLEX vector and expressed in E.coli GI724. The recombinant fusion protein was identified by SDS-PAGE and Western blot, and the SN was characterized by TDA method. Results and C onclusions: The fusion protein was effectively expressed. Its relativ e molecular weight was… CONTINUE READING