• Corpus ID: 13397345

Expression of the cell cycle in sperm of Arabidopsis: implications for understanding patterns of gametogenesis and fertilization in plants and other eukaryotes.

  title={Expression of the cell cycle in sperm of Arabidopsis: implications for understanding patterns of gametogenesis and fertilization in plants and other eukaryotes.},
  author={William E. Friedman},
  volume={126 5},
  • W. Friedman
  • Published 1 March 1999
  • Environmental Science, Biology
  • Development
The relationship between developmental events and the cell cycle was examined in sperm of Arabidopsis thaliana. [] Key Result Sperm of Arabidopsis rapidly enter the S (synthesis) phase of the cell cycle after inception from mitosis of the generative cell. Sperm in pollen grains within anthers continue to synthesize DNA, and at the time of pollination, contain approximately 1.5C DNA. Following pollination, sperm continue through the S phase of the cell cycle during pollen tube growth.

Figures and Tables from this paper

Relationship between nuclear DNA-content of sperm cells and the timing of events in the cell cycle of Brassica campestris L. (Brassicaceae)

The results indicate that during development, sperm cells of B. campestris have passed the G 1 phase of the cell cycle and entered the S phase, presumably then fusing with egg cells at a level of 2C, as is characteristic of G 2 type fertilization in angiosperms.

Relationship between double fertilization and the cell cycle in male and female gametes of tobacco

Failure to achieve an optimized system for in vitro fertilization in Nicotiana may reflect the challenges of achieving cell cycle synchrony in gametes isolated from pollen tubes.

Comparative Transcriptomics of Arabidopsis Sperm Cells

Functional classifications of genes with enriched expression in sperm cells showed that DNA repair, ubiquitin-mediated proteolysis, and cell cycle progression are overrepresented Gene Ontology categories, and analysis of the small RNA and DNA methylation pathways suggests that distinct mechanisms might be involved in regulating the epigenetic state of the paternal genome.

Migration of sperm cells during pollen tube elongation in Arabidopsis thaliana: behavior during transport, maturation and upon dissociation of male germ unit associations

The promoter sequence of sperm-expressed gene, PzIPT isolated from the Svn (sperm associated with the vegetative nucleus) of Plumbago zeylanica, was fused to a green fluorescent protein (GFP)

Transcriptomics of Arabidopsis sperm cells at single-cell resolution

A detailed protocol for isolation of single sperm cells from growing pollen tubes and transcriptome analysis to study variation in gene expression between sperm cells is presented.

Chromatin assembly factor 1 regulates the cell cycle but not cell fate during male gametogenesis in Arabidopsis thaliana

It is shown in Arabidopsis thaliana that loss of activity of the Chromatin assembly factor 1 (CAF1) pathway causes delay and arrest of the cell cycle during pollen development, suggesting that pollen cell fate is independent from cell cycle regulation.

Comparative Transcriptomics of Arabidopsis Sperm Cells1[C][W]

Comparative analysis of the sperm cell transcriptome with those of representative sporophytic tissues and of pollen showed that sperm has a distinct and diverse transcriptional profile, andAnalysis of the small RNA and DNA methylation pathways suggests that distinct mechanisms might be involved in regulating the epigenetic state of the paternal genome.

Nuclear DNA replicates during zygote development in Arabidopsis and Torenia fournieri.

This study reassessed the phase of cell-cycle arrest in the gametes of two species, Arabidopsis (Arabidopsis thaliana) and Torenia fournieri, and found that the replication of nuclear DNA did not occur during gamete development but during zygote development, revealing that the gamets of these species have a haploid nuclear DNA content before fertilization.

Male Germ Line Development in Arabidopsis. duo pollen Mutants Reveal Gametophytic Regulators of Generative Cell Cycle Progression1[w]

These results identify two essential gametophytic loci required for progression through different phases of the generative cell cycle, providing the first evidence to the authors' knowledge for genetic regulators of male germ line development in flowering plants.



Cell cycle regulatory genes from maize are differentially controlled during fertilization and first embryonic cell division

All three cyclins examined are transcribed de novo after fertilization, indicating a different level of embryonic cell cycle regulation in plants than what is commonly found in animals where early embryonic cell divisions are driven by maternally supplied cyclin mRNA.

Pollen-pistil interactions and developmental regulation of pollen tube growth in Arabidopsis

The acquisition of reproductive function was found to involve, in addition to the induction of a variety of stimulatory signals, a heretofore unrecognized developmental restriction in the capacity of epidermal surfaces of the flower to support pollen tube growth.

Cell biology of embryo sac development in Arabidopsis

The cytoskeleton during megasporogenesis, megagametogenesis and early embryogenesis is investigated, recognizing a particular need to compile basic information on Arabidopsis thaliana, this plant has been the subject of observations.

Cell Cycle Control in Arabidopsis

While expression of oneArabidopsisCDK gene was positively correlated with the competence of cells to divide, expression of a mitotic-like cyclin,cyc1At, was almost exclusively confined to dividing cells and appears to be an early stage in the response of plant cells to external and internal stimuli.

Dynamics of Nuclear DNA Quantities during Zygote Development in Barley.

Results indicate that the egg and sperm nuclei are at the 1C DNA level (basic haploid amount) at the time of karyogamy, which is fundamental to a better understanding of fertilization and zygote maturation in flowering plants.

Double Fertilization in Gnetum gnemon: The Relationship between the Cell Cycle and Sexual Reproduction.

The results show definitively a pattern of G2 karyogamy in G. gnemon, a nonflowering seed plant and member of the Gnetales that expresses a rudimentary pattern of double fertilization that results in the formation of two zygotes per pollen tube.

TETRASPORE is required for male meiotic cytokinesis in Arabidopsis thaliana.

Four mutant alleles of TETRASPORE (TES), a gene essential for microsporocyte cytokinesis in Arabidopsis thaliana, are described, which reveal a gene specific to male meiosis and aid investigation of a wide range of processes in pollen development and function.

The structure of the transmitting tissue of Arabidopsis thaliana (L.) and the path of pollen tube growth

Yariv phenylglycoside and monoclonal antibodies to arabinogalactan proteins (AGPs) provide evidence that AGPs are a component of the septum epidermal ECM, and this is the first description of pollen tube growth in vivo in Arabidopsis thaliana.


The timing of replication and division of the Chlamydomonas Ehrenberg nucleus in the vegetative cell cycle and at gametogenesis was examined, using fluorescence microspectrophotometry with two

Regulation of Cell Division in Arabidopsis

Although much remains to be studied concerning upstream regulators of these genes, the successful introduction of mutant CDKs into plants demonstrates the potential of using such an approach to intentionally modulate the plant cell cycle and development.