Expression of the aphthovirus RNA polymerase gene in Escherichia coli and its use together with other bioengineered nonstructural antigens in detection of late persistent infections.

@article{Neitzert1991ExpressionOT,
  title={Expression of the aphthovirus RNA polymerase gene in Escherichia coli and its use together with other bioengineered nonstructural antigens in detection of late persistent infections.},
  author={Erika Neitzert and Ewald Beck and Paulo Andrade de Mello and Iva Gomes and Ingrid Evelyn Bergmann},
  journal={Virology},
  year={1991},
  volume={184 2},
  pages={799-804}
}
A plasmid has been constructed containing the DNA sequences that direct the expression of the aphthovirus RNA-dependent RNA polymerase (virus infection-associated antigen, VIAA) in its native form. The aphthovirus polypeptide was designed to contain only a single additional amino acid, the N-terminal methionine. The recombinant protein has been purified and… CONTINUE READING