Expression of the Variable Region Genes of the Monoclonal Antibodies Against Metal-bound Tetrapeptide in E. coli.

@article{Han1996ExpressionOT,
  title={Expression of the Variable Region Genes of the Monoclonal Antibodies Against Metal-bound Tetrapeptide in E. coli.},
  author={L P Han and Li-Ying Shen and C Chen and Cheng-Zhi Su},
  journal={Sheng wu hua xue yu sheng wu wu li xue bao Acta biochimica et biophysica Sinica},
  year={1996},
  volume={28 6},
  pages={583-589}
}
The variable region genes of the light and heavy chains obtained from three stems of McAb against metal-bound tetrapeptide were joined into a single chain by a linker. A 39 bp fragment of the N-terminal of CGRP was joined to the C-terminal of the heavy chain to constitute the Lv-linker-Hv-CGRP single chain gene which was cloned into the vector pTC01 and expressed in E. coli 71/18. The molecular weight of the expressed product was approximately 26 kD as shown by SDS-PAGE. Its expression level… CONTINUE READING

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